{"id":"d0c6c1ce-079f-5263-9e86-18451ca493d7","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:formate-mthfd1-assimilation","predicate":"assimilates","statement":"Provided carbon-13 formate entered cytosolic 10-formyl-THF and ATP in wild-type HEK293T cells through the MTHFD1 assimilation route.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"032739cc-9e8e-5294-92c5-7d3fb7eba4a4","mechanism_event_label":"Cells incorporate formate carbon into purine nucleotides.","subject":{"id":"ae1cf62b-2ef9-52d7-bbf6-6817abb50d98","slug":"mthfd1","display_name":"Human MTHFD1","entity_type_key":"protein"},"object":{"id":"1fc1fa42-fcb5-5983-9a31-114703dc64c4","slug":"formate","display_name":"Formate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"032739cc-9e8e-5294-92c5-7d3fb7eba4a4","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:formate-mthfd1-assimilation-event","event_type":"biochemical_relationship","label":"Cells incorporate formate carbon into purine nucleotides.","description":"Provided carbon-13 formate entered cytosolic 10-formyl-THF and ATP in wild-type HEK293T cells through the MTHFD1 assimilation route.","status":"provisional","compartment":{"slug":"cytosol","display_name":"Cytosol"},"participants":[{"entity":{"id":"ec73b364-a2ea-5c4a-bcea-a54aeebf57d1","slug":"10-formyltetrahydrofolate","display_name":"10-Formyltetrahydrofolate","entity_type_key":"small_molecule"},"role":"assimilated folate intermediate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"labeled purine nucleotide","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ae1cf62b-2ef9-52d7-bbf6-6817abb50d98","slug":"mthfd1","display_name":"Human MTHFD1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"1fc1fa42-fcb5-5983-9a31-114703dc64c4","slug":"formate","display_name":"Formate","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Formate rescue and metabolomics","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Carbon-13 formate tracer in wild-type cells; Figure 2d.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Tracing supports pathway use but is not an isolated enzyme assay or dietary recommendation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Cells incorporate formate carbon into purine nucleotides.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ducker-2016] Reversal of Cytosolic One-Carbon Flux Compensates for Loss of the Mitochondrial Folate Pathway (2016). https://pubmed.ncbi.nlm.nih.gov/27211901/ DOI: 10.1016/j.cmet.2016.04.016","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"HEK293T cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9842e294-1966-514f-8976-5ca7fc944458","evidence_kind":"source_excerpt","locator":"Lines 911-921","start_line":911,"end_line":921,"excerpt":"### formate-mthfd1-assimilation\nProvided carbon-13 formate entered cytosolic 10-formyl-THF and ATP in wild-type HEK293T cells through the MTHFD1 assimilation route.\nCondition category: normal\nnutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Cells incorporate formate carbon into purine nucleotides.\norganism: Homo sapiens\ntissue_or_cell_type: HEK293T cells\nexperimental_model: Formate rescue and metabolomics\nlimitations: Tracing supports pathway use but is not an isolated enzyme assay or dietary recommendation.\nexposure: Carbon-13 formate tracer in wild-type cells; Figure 2d.\n[ducker-2016] Reversal of Cytosolic One-Carbon Flux Compensates for Loss of the Mitochondrial Folate Pathway (2016). https://pubmed.ncbi.nlm.nih.gov/27211901/ DOI: 10.1016/j.cmet.2016.04.016","model_system":"Formate rescue and metabolomics","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [ducker-2016] Reversal of Cytosolic One-Carbon Flux Compensates for Loss of the Mitochondrial Folate Pathway (2016). https://pubmed.ncbi.nlm.nih.gov/27211901/ DOI: 10.1016/j.cmet.2016.04.016","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f4ce1a62-9582-5f7a-84f5-a23d0e1bfc68","stable_key":"import-ec174d5a-4903-5745-8646-df0e9d4265e8","title":"Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e564d43989ece1006c95cd0748e9af6fe369074599a2eebba0a99ebff864b0dd","revision_id":"76674a33-b2a1-5e41-b71b-44399038ff7c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}