{"id":"d0be9317-8860-592b-a7f6-bc281ac4bfa1","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-neuro-ddc-internal-aldimine","predicate":"forms-internal-aldimine-with","statement":"Human holoDDC crystallography resolved an internal PLP aldimine at 1.9 angstrom resolution.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"5af6a2fe-8acd-594d-b782-26e770b6b6b3","mechanism_event_label":"The active B6 cofactor is chemically attached inside DDC.","subject":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"object":{"id":"cdc3fa8b-c680-5b62-bac0-f4d9b3baed95","slug":"ddc","display_name":"Human aromatic L-amino acid decarboxylase / DDC","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"5af6a2fe-8acd-594d-b782-26e770b6b6b3","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-neuro-ddc-internal-aldimine-event","event_type":"biochemical_relationship","label":"The active B6 cofactor is chemically attached inside DDC.","description":"Human holoDDC crystallography resolved an internal PLP aldimine at 1.9 angstrom resolution.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"role":"cofactor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"cdc3fa8b-c680-5b62-bac0-f4d9b3baed95","slug":"ddc","display_name":"Human aromatic L-amino acid decarboxylase / DDC","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Recombinant human DDC structures, SAXS and molecular dynamics","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Crystallized purified holoenzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Structure describes a catalytic state, not a dietary dose requirement.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b6","display_name":"Vitamin B6","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The active B6 cofactor is chemically attached inside DDC.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[bisello-2023-ddc] Human aromatic amino acid decarboxylase is an asymmetric and flexible enzyme: Implication in aromatic amino acid decarboxylase deficiency (2023). https://pmc.ncbi.nlm.nih.gov/articles/PMC10382914/ DOI: 10.1002/pro.4732","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified recombinant protein; no intact tissue","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"652c7654-0ace-5559-b05a-eaa46240b257","evidence_kind":"source_excerpt","locator":"Lines 1035-1045","start_line":1035,"end_line":1045,"excerpt":"### b6-neuro-ddc-internal-aldimine\nHuman holoDDC crystallography resolved an internal PLP aldimine at 1.9 angstrom resolution.\nCondition category: normal\nnutrient_topic: Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The active B6 cofactor is chemically attached inside DDC.\norganism: Homo sapiens\ntissue_or_cell_type: Purified recombinant protein; no intact tissue\nexperimental_model: Recombinant human DDC structures, SAXS and molecular dynamics\nlimitations: Structure describes a catalytic state, not a dietary dose requirement.\nexposure: Crystallized purified holoenzyme.\n[bisello-2023-ddc] Human aromatic amino acid decarboxylase is an asymmetric and flexible enzyme: Implication in aromatic amino acid decarboxylase deficiency (2023). https://pmc.ncbi.nlm.nih.gov/articles/PMC10382914/ DOI: 10.1002/pro.4732","model_system":"Recombinant human DDC structures, SAXS and molecular dynamics","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [bisello-2023-ddc] Human aromatic amino acid decarboxylase is an asymmetric and flexible enzyme: Implication in aromatic amino acid decarboxylase deficiency (2023). https://pmc.ncbi.nlm.nih.gov/articles/PMC10382914/ DOI: 10.1002/pro.4732","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"251773bb-16f5-5903-b135-db4a61d9dec4","stable_key":"import-1310afbd-6010-586e-805d-551d846da421","title":"Vitamin B6: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"ef0019b344b2219220f801a84d0d138ff6880c1be1a59540d9034bfa4334f61e","revision_id":"cac3555f-48af-5c52-a84e-add4482c87fb","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}