{"id":"d0a85c09-813c-5eb2-8ab8-096f78f625c7","stable_key":"066fc1e8-fefc-5be0-9905-938b10b89691:rmdn3-kd-a39-viability","predicate":"has_contextual_experimental_effect","statement":"RMDN3 knockdown reduced HeLa cell viability during SLC25A39 silencing.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"ac6dbfb5-dea7-5d28-8f32-38ee65f7062c","mechanism_event_label":"rmdn3 kd a39 viability","subject":{"id":"a989c84d-68b6-5f9f-bd27-be676ec060fd","slug":"human-rmdn3-knockdown","display_name":"RMDN3 knockdown in human cells","entity_type_key":"protein_state"},"object":{"id":"50057ef2-87c5-5737-9f88-73835eb0e986","slug":"human-hela-cell-viability","display_name":"HeLa cell viability readout","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"ac6dbfb5-dea7-5d28-8f32-38ee65f7062c","stable_key":"066fc1e8-fefc-5be0-9905-938b10b89691:rmdn3-kd-a39-viability","event_type":"experimental_result","label":"rmdn3 kd a39 viability","description":"RMDN3 knockdown reduced HeLa cell viability during SLC25A39 silencing.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a989c84d-68b6-5f9f-bd27-be676ec060fd","slug":"human-rmdn3-knockdown","display_name":"RMDN3 knockdown in human cells","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"50057ef2-87c5-5737-9f88-73835eb0e986","slug":"human-hela-cell-viability","display_name":"HeLa cell viability readout","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"543c9bd9-03a3-5a08-b1f7-08184225765e","slug":"human-a39-knockdown-hela","display_name":"SLC25A39 knockdown in HeLa cells","entity_type_key":"protein_state"},"role":"experimental background","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"access_level","value_text":"selected_primary_main_text_and_legends; cached web/indexed passages, not complete supplement review","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_cache","value_text":"artifacts/discovery-research/round4-sources/contact-2025-indexed-passages.json; SHA256 9222f94099520a9b5e9ee8b7f30a1b4ae261735d37462b13d7ca3d0ecc8792d6","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"HeLa; MERBiT reporter derivative where stated","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Combined silencing; CCK-8 viability assay; exact supplementary dose and timing unresolved","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Selected primary main-text Results and figure legends reviewed; supplementary images not independently inspected. These two papers share authors and are not independent replication. They do not test whether SLC25A39-loss-induced contacts increase HMOX2-dependent iron delivery. ICP-MS measures total iron, not labile iron or transport flux. Contact loss affecting an outcome does not establish that increasing contacts increases it. Ubiquitination/activity readouts do not establish direct enzymatic activation or the outer-membrane iron transport machinery. 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These two papers share authors and are not independent replication. They do not test whether SLC25A39-loss-induced contacts increase HMOX2-dependent iron delivery. ICP-MS measures total iron, not labile iron or transport flux. Contact loss affecting an outcome does not establish that increasing contacts increases it. Ubiquitination/activity readouts do not establish direct enzymatic activation or the outer-membrane iron transport machinery. No clinical or pulmonary-endothelial effect is inferred.","model_system":"Human HeLa cells","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Authored paraphrase with primary location; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e52836df-b22a-51c4-9ab4-6b258a554969","stable_key":"import-066fc1e8-fefc-5be0-9905-938b10b89691","title":"Glutathione, ER-mitochondria contacts, and iron: observed components","document_type":"imported_text","citation_label":"Targeted primary-literature curation, 2026-09-20. DOIs 10.1038/s41467-025-56666-4 and 10.1038/s41556-026-01974-0.","file_path":"","sha256":"381747aa54ebbd40dfc3d830264e0231a09c3e417ad896a591dffc10dcdc64f4","revision_id":"d679b06d-a889-5aba-b69b-f5c07d393c24","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}