{"id":"d09a4928-e0be-5877-a75d-d2a2cd45dfba","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-paox-spermine-null","predicate":"poor_substrate_activity_on","statement":"The recombinant human PAOX preparation did not efficiently oxidize unacetylated spermine.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"ad2d826a-9004-54b5-a832-2db3458d0895","mechanism_event_label":"Acetylation changes substrate recognition.","subject":{"id":"80b7a4f6-c2c5-5ee1-8fab-c3500bb5c79c","slug":"paox","display_name":"Human N1-acetylpolyamine oxidase / PAOX","entity_type_key":"protein"},"object":{"id":"ded80d0b-9076-5034-9e87-cd38f7dafae5","slug":"spermine","display_name":"Spermine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"ad2d826a-9004-54b5-a832-2db3458d0895","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-paox-spermine-null-event","event_type":"observed_relationship","label":"Acetylation changes substrate recognition.","description":"The recombinant human PAOX preparation did not efficiently oxidize unacetylated spermine.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"80b7a4f6-c2c5-5ee1-8fab-c3500bb5c79c","slug":"paox","display_name":"Human N1-acetylpolyamine oxidase / PAOX","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ded80d0b-9076-5034-9e87-cd38f7dafae5","slug":"spermine","display_name":"Spermine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7014993c-468f-5c6b-ab04-80c87f9dfd9f","slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified enzyme comparison.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Do not substitute PAOX for SMOX in the graph.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Spermidine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Acetylation changes substrate recognition.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Properties of recombinant human N1-acetylpolyamine oxidase (hPAO): potential role in determining drug sensitivity. · 2005 · https://pubmed.ncbi.nlm.nih.gov/15791459/ · DOI 10.1007/s00280-004-0936-5","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"70da36ce-a8ac-5e79-b2bf-e6afc009a8a6","evidence_kind":"source_excerpt","locator":"Lines 110-116","start_line":110,"end_line":116,"excerpt":"## spermidine-paox-spermine-null\nAcetylation changes substrate recognition.\nThe recombinant human PAOX preparation did not efficiently oxidize unacetylated spermine.\nModel: Purified enzyme comparison.\nLimitations: Do not substitute PAOX for SMOX in the graph.\nEvidence access: Primary abstract\nProperties of recombinant human N1-acetylpolyamine oxidase (hPAO): potential role in determining drug sensitivity. · 2005 · https://pubmed.ncbi.nlm.nih.gov/15791459/ · DOI 10.1007/s00280-004-0936-5","model_system":"Purified enzyme comparison.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4348f3f8-0180-58a3-b61c-55d03bb322d2","stable_key":"import-88eb7407-f147-558b-889b-0c89c4e0aa4a","title":"Spermidine: biosynthesis, hypusination, transport and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"8eec8784bc2bd0384cfd53c6603c4ced8e53c227d9ab29ed85594c43e34c68ef","revision_id":"7c421a18-4526-59f6-b84e-4be533bc35ac","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}