{"id":"d06dbcb6-29bc-5388-948f-0e5eaab4b819","stable_key":"research:txn2-regenerates-prdx3","predicate":"reduces","statement":"Reduced TXN2 regenerates the peroxide-reducing form of PRDX3.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"literature_reviewed:supported_interpretation","direction":"neutral","is_public":true,"mechanism_event_id":"e4e1dd7e-d85e-5451-a333-2719ce14c5bc","mechanism_event_label":"Thioredoxin recharges a mitochondrial peroxide-removal enzyme.","subject":{"id":"f1da8147-06b9-543e-af26-f1a97ace4f93","slug":"txn2","display_name":"TXN2","entity_type_key":"protein"},"object":{"id":"ebb7ab23-2e3c-5334-8941-68f95c5879ea","slug":"prdx3","display_name":"PRDX3","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"e4e1dd7e-d85e-5451-a333-2719ce14c5bc","stable_key":"research:txn2-regenerates-prdx3","event_type":"redox-transfer","label":"Thioredoxin recharges a mitochondrial peroxide-removal enzyme.","description":"Reduced TXN2 regenerates the peroxide-reducing form of PRDX3.","status":"active","compartment":{"slug":"mitochondrial-matrix","display_name":"Mitochondrial matrix"},"participants":[{"entity":{"id":"f1da8147-06b9-543e-af26-f1a97ace4f93","slug":"txn2","display_name":"TXN2","entity_type_key":"protein"},"role":"electron-donor","stoichiometry":null,"state_label":"reduced to oxidized","sequence_order":0,"notes":""},{"entity":{"id":"ebb7ab23-2e3c-5334-8941-68f95c5879ea","slug":"prdx3","display_name":"PRDX3","entity_type_key":"protein"},"role":"redox-substrate","stoichiometry":null,"state_label":"oxidized to reduced","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"cell_type","value_text":"cardiac mitochondria","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Redox-state measurements","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"TXN2 and PRDX3 are not selenoproteins.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"mouse and guinea pig","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"2acde955-bf67-544b-b0ef-2e0fd4a12666","evidence_kind":"curated_literature_summary","locator":"lines 618-628","start_line":618,"end_line":628,"excerpt":"## txn2-regenerates-prdx3\n\nThioredoxin recharges a mitochondrial peroxide-removal enzyme.\n\nReduced TXN2 regenerates the peroxide-reducing form of PRDX3.\n\nOrganism: mouse and guinea pig\nCell type: cardiac mitochondria\nExperimental model: Redox-state measurements\nLimitations: TXN2 and PRDX3 are not selenoproteins.\nPrimary reference: [Thioredoxin Reductase-2 Is Essential for Keeping Low Levels of H2O2 Emission from Isolated Heart Mitochondria](https://pubmed.ncbi.nlm.nih.gov/21832082/)","model_system":"Redox-state measurements","directness":"author_interpretation","verification_status":"secondary_verified","notes":"Curated summary; inspect the linked primary papers for original methods and results.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f892f13-06ea-5199-a33c-a703f35c80ae","stable_key":"selenium-research-2026-09-17","title":"Selenium: literature corrections and mechanism additions","document_type":"curated_literature_review","citation_label":"Metabolic Ledger literature curation, 17 September 2026; primary papers linked individually","file_path":"","sha256":"0b818b10c1c7120e5caf7f4d4019d7bd025d745692e424f515d3ef903c9ab7f3","revision_id":"80984e03-5f0f-5877-8094-afef7637444e","review_status":"secondary_verified","notes":"Secondary curated summaries of primary experiments, with explicit models and limitations. Not archived primary full text."}}],"relations":[],"conflicts":[],"corrections":[],"research":{"topic":"mitochondrial-redox","plain_language":"Thioredoxin recharges a mitochondrial peroxide-removal enzyme.","evidence_scope":"supported_interpretation","papers":[{"key":"stanley-2011-txnrd2-relay","title":"Thioredoxin Reductase-2 Is Essential for Keeping Low Levels of H2O2 Emission from Isolated Heart Mitochondria","url":"https://pubmed.ncbi.nlm.nih.gov/21832082/","doi":"10.1074/jbc.M111.284612","year":2011,"model":"Mouse and guinea-pig cardiac mitochondria and cardiomyocytes","summary":"Tests the mitochondrial thioredoxin/peroxiredoxin peroxide-removal circuit using redox and functional measurements."}]}}