{"id":"d049f5da-033d-53ee-bf33-9b3442d12f73","stable_key":"7edf94bb-95c8-5234-9161-9eb338bb9b36:boron-myoblast-mechanics","predicate":"increases","statement":"Soluble boron increased retrograde actin flow and traction forces in C2C12 myoblasts on fibronectin hydrogels, with responses also dependent on hydrogel stiffness.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"613d7208-12d5-5b7f-9c00-3915f9dda418","mechanism_event_label":"The boron exposure changed how cultured muscle precursor cells pulled on their surroundings.","subject":{"id":"cfbf4db0-5a59-56fe-a2b8-2176eac27237","slug":"borax","display_name":"Sodium tetraborate / borax","entity_type_key":"chemical_species"},"object":{"id":"bba439f4-a33f-54a2-931c-7ce7cb67c6fc","slug":"retrograde-actin-flow","display_name":"Retrograde actin flow","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"613d7208-12d5-5b7f-9c00-3915f9dda418","stable_key":"7edf94bb-95c8-5234-9161-9eb338bb9b36:boron-myoblast-mechanics-event","event_type":"biochemical_relationship","label":"The boron exposure changed how cultured muscle precursor cells pulled on their surroundings.","description":"Soluble boron increased retrograde actin flow and traction forces in C2C12 myoblasts on fibronectin hydrogels, with responses also dependent on hydrogel stiffness.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"d4cb804f-b7de-5d1b-8dbe-085d58fa29a6","slug":"mouse-slc4a11","display_name":"Mouse Slc4a11 / NaBC1","entity_type_key":"protein"},"role":"implicated_protein","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"cfbf4db0-5a59-56fe-a2b8-2176eac27237","slug":"borax","display_name":"Sodium tetraborate / borax","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"bba439f4-a33f-54a2-931c-7ce7cb67c6fc","slug":"retrograde-actin-flow","display_name":"Retrograde actin flow","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/boron-research/40270477.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"e55fb244b3c6f4adc9c4b1ab4ed2e8bb2a866b01bc9b50b16ed85e1f4737aeab\", \"start_char\": 0, \"end_char\": 997, \"text_sha256\": \"e55fb244b3c6f4adc9c4b1ab4ed2e8bb2a866b01bc9b50b16ed85e1f4737aeab\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Substrate-stiffness experiments, traction measurements and Slc4a11 silencing","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Soluble boron supplied as borax; reported experimental concentrations 0.59 and 1.47 mM; variable substrate stiffness","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Protein-dependence of a cell-mechanics phenotype does not demonstrate transmembrane borate transport. 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The authors use the historical transporter label; human transport remains disputed. Matrix coating and exposure are essential context.\nexposure: Soluble boron supplied as borax; reported experimental concentrations 0.59 and 1.47 mM; variable substrate stiffness\nevidence_span: {\"source_cache\": \"artifacts/boron-research/40270477.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"e55fb244b3c6f4adc9c4b1ab4ed2e8bb2a866b01bc9b50b16ed85e1f4737aeab\", \"start_char\": 0, \"end_char\": 997, \"text_sha256\": \"e55fb244b3c6f4adc9c4b1ab4ed2e8bb2a866b01bc9b50b16ed85e1f4737aeab\"}\n[boron-p40270477] NaBC1 Boron Transporter Enables Myoblast Response to Substrate Rigidity via Fibronectin-Binding Integrins. 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