{"id":"d0487c54-163d-570d-ae81-dcd340212394","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-htr4-loss","predicate":"knockout_abolishes","statement":"Htr4 knockout or receptor antagonism removed the secretory response to tryptamine.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"a5e19321-db82-5ed6-82e3-1eb877aea88b","mechanism_event_label":"The metabolite could not produce this response without its receptor.","subject":{"id":"fd074f31-c694-5703-9237-0544bf024341","slug":"mouse-htr4","display_name":"Mouse serotonin receptor 4 / Htr4","entity_type_key":"protein"},"object":{"id":"850b1f47-62fb-535f-b1e1-3b250e7b6c25","slug":"mouse-tryptamine-secretion","display_name":"Mouse colonic ionic and fluid secretion after tryptamine","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"a5e19321-db82-5ed6-82e3-1eb877aea88b","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-htr4-loss-event","event_type":"observed_relationship","label":"The metabolite could not produce this response without its receptor.","description":"Htr4 knockout or receptor antagonism removed the secretory response to tryptamine.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"fd074f31-c694-5703-9237-0544bf024341","slug":"mouse-htr4","display_name":"Mouse serotonin receptor 4 / Htr4","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"850b1f47-62fb-535f-b1e1-3b250e7b6c25","slug":"mouse-tryptamine-secretion","display_name":"Mouse colonic ionic and fluid secretion after tryptamine","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"769339cb-213b-559e-acc0-07ed00368b94","slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e009ad82-c5b9-5dba-96dd-b7e85060da2c","slug":"tryptamine","display_name":"Tryptamine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse knockout and pharmacological blockade.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not a universal receptor requirement for every action of tryptamine.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Tryptophan collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The metabolite could not produce this response without its receptor.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Gut Microbiota-Produced Tryptamine Activates an Epithelial G-Protein-Coupled Receptor to Increase Colonic Secretion. · 2018 · https://pubmed.ncbi.nlm.nih.gov/29902441/ · DOI 10.1016/j.chom.2018.05.004","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"55b5dfe3-395b-5cca-9917-ed42a441d94c","evidence_kind":"source_excerpt","locator":"Lines 570-576","start_line":570,"end_line":576,"excerpt":"## tryptophan-htr4-loss\nThe metabolite could not produce this response without its receptor.\nHtr4 knockout or receptor antagonism removed the secretory response to tryptamine.\nModel: Mouse knockout and pharmacological blockade.\nLimitations: Not a universal receptor requirement for every action of tryptamine.\nEvidence access: Primary abstract\nGut Microbiota-Produced Tryptamine Activates an Epithelial G-Protein-Coupled Receptor to Increase Colonic Secretion. · 2018 · https://pubmed.ncbi.nlm.nih.gov/29902441/ · DOI 10.1016/j.chom.2018.05.004","model_system":"Mouse knockout and pharmacological blockade.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"73f9d3e7-fdc9-5418-8f3c-f4ef145f6efa","stable_key":"import-584c58f5-ab9f-53f3-97a9-55783db943b0","title":"Tryptophan: transport, protein synthesis, neuroactive metabolites, NAD and microbial pathways (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"7dfd20063b91900cb5c6ad675e3e675b7e25ef0fb8304eabac6f263cc5518284","revision_id":"bfafd789-fdf5-521f-bd28-c662e01ec1ea","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}