{"id":"cfe0feb8-b34d-55ff-8d8f-a143d8de09ea","stable_key":"8600a864-6653-52c7-9718-1362c5af4577:gamma-nonalactone-cocaine-range-limit","predicate":"no_detected_protection","statement":"Gamma-nonalactone at 75-100 micromolar did not prevent the cocaine-associated locomotion decrease.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"b88434fd-36aa-519a-9019-e43f1870e1f5","mechanism_event_label":"Gamma-nonalactone at 75-100 micromolar did not prevent the cocaine-associated locomotion decrease.","subject":{"id":"2b85fa4a-5c91-5de9-aead-a443bc4394af","slug":"gamma-nonalactone","display_name":"Gamma-nonalactone","entity_type_key":"small_molecule"},"object":{"id":"d490dd91-29b8-5008-b933-d21336d11df7","slug":"dugesia-tigrina-locomotion","display_name":"Locomotion of Dugesia tigrina planarians","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"b88434fd-36aa-519a-9019-e43f1870e1f5","stable_key":"8600a864-6653-52c7-9718-1362c5af4577:gamma-nonalactone-cocaine-range-limit-event","event_type":"observed_relationship","label":"Gamma-nonalactone at 75-100 micromolar did not prevent the cocaine-associated locomotion decrease.","description":"Gamma-nonalactone at 75-100 micromolar did not prevent the cocaine-associated locomotion decrease.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2b85fa4a-5c91-5de9-aead-a443bc4394af","slug":"gamma-nonalactone","display_name":"Gamma-nonalactone","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d490dd91-29b8-5008-b933-d21336d11df7","slug":"dugesia-tigrina-locomotion","display_name":"Locomotion of Dugesia tigrina planarians","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"46b2f8a0-01a9-5041-b13b-2c46982873d6","slug":"cocaine","display_name":"Cocaine","entity_type_key":"drug"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Gamma-nonalactone 75-100 micromolar with cocaine 200 micromolar","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"10 min incubation followed by 5 min recording","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary full-text methods/results inspected; PubMed metadata where indexed.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Dugesia tigrina planarians","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Loss of protection at higher concentrations is not a scientific conflict with the lower-dose response; it occurs in the same study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Gamma-nonalactone flavor-compound chapter; nutrient and drug interactions retain their experimental settings.","comparator":null,"unit":null,"notes":"","entity":{"slug":"gamma-nonalactone","display_name":"Gamma-nonalactone","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Dugesia tigrina planarians","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Gamma-nonalactone at 75-100 micromolar did not prevent the cocaine-associated locomotion decrease.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Minimal structural requirements of alkyl γ-lactones capable of antagonizing the cocaine-induced motility decrease in planarians. (2011). https://pubmed.ncbi.nlm.nih.gov/21878350/ DOI: 10.1016/j.pbb.2011.08.013","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"Whole-worm bath co-exposure","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Grid-crossing locomotion assay","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f5b6f835-1bce-5cfe-b9d8-1f9fc0076483","evidence_kind":"source_excerpt","locator":"Lines 213-222","start_line":213,"end_line":222,"excerpt":"## gamma-nonalactone-cocaine-range-limit\nGamma-nonalactone at 75-100 micromolar did not prevent the cocaine-associated locomotion decrease.\nModel/species: Dugesia tigrina planarians\nTissue: Grid-crossing locomotion assay\nExposure: Gamma-nonalactone 75-100 micromolar with cocaine 200 micromolar\nRoute: Whole-worm bath co-exposure\nDuration: 10 min incubation followed by 5 min recording\nLimits: Loss of protection at higher concentrations is not a scientific conflict with the lower-dose response; it occurs in the same study.\nPrimary reference: Minimal structural requirements of alkyl γ-lactones capable of antagonizing the cocaine-induced motility decrease in planarians. (2011). https://pubmed.ncbi.nlm.nih.gov/21878350/ DOI: 10.1016/j.pbb.2011.08.013\nAccess: Primary full-text methods/results inspected; PubMed metadata where indexed.","model_system":"Dugesia tigrina planarians","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span with primary citation; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"73777c54-95a4-5b07-9d14-d4dd31949fb1","stable_key":"import-8600a864-6653-52c7-9718-1362c5af4577","title":"Gamma-nonalactone: mechanisms, molecular forms and cross-actor connections (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted curation of eight primary studies. Study-specific citations, negative findings and limitations retained. 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