{"id":"cfc56a06-fcc2-5252-bbec-ca18d62f70bf","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:fkbp65-plod2-dimer","predicate":"supports_assembly_of","statement":"FKBP65 supports LH2/PLOD2 dimer formation in the tested collagen-producing cell systems.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"6cab1bb2-1290-58ce-8aeb-07f2165fa776","mechanism_event_label":"A helper protein helps assemble the collagen-modifying enzyme.","subject":{"id":"953d708b-9d69-5aad-b3c7-0e5be74d3a9a","slug":"fkbp10","display_name":"FKBP10","entity_type_key":"protein"},"object":{"id":"21db67f1-4792-5d3d-b2d0-9b204d060678","slug":"plod2-dimer","display_name":"PLOD2 dimer","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"6cab1bb2-1290-58ce-8aeb-07f2165fa776","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:fkbp65-plod2-dimer-event","event_type":"biochemical_relationship","label":"A helper protein helps assemble the collagen-modifying enzyme.","description":"FKBP65 supports LH2/PLOD2 dimer formation in the tested collagen-producing cell systems.","status":"provisional","compartment":{"slug":"endoplasmic-reticulum","display_name":"Endoplasmic reticulum"},"participants":[{"entity":{"id":"953d708b-9d69-5aad-b3c7-0e5be74d3a9a","slug":"fkbp10","display_name":"FKBP10","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"21db67f1-4792-5d3d-b2d0-9b204d060678","slug":"plod2-dimer","display_name":"PLOD2 dimer","entity_type_key":"protein_complex"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"e7ea826f-6609-5b8a-887c-0609970a06fe","slug":"plod2","display_name":"PLOD2","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Human collagen-producing fibroblasts and expression constructs; predominantly LH2B dimerization/interaction assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This is an experimentally scoped assembly mechanism; free lysine supplementation was not tested.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Human","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A helper protein helps assemble the collagen-modifying enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[plod2-fkbp65-2016] Disentangling mechanisms involved in collagen pyridinoline cross-linking: The immunophilin FKBP65 is critical for dimerization of lysyl hydroxylase 2 (2016). https://pmc.ncbi.nlm.nih.gov/articles/PMC4932945/ DOI: 10.1073/pnas.1600074113","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Not specified as a whole tissue; see experimental model.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6a611feb-c237-51c6-a9e6-1c0e053ea110","evidence_kind":"source_excerpt","locator":"Lines 407-415","start_line":407,"end_line":415,"excerpt":"### fkbp65-plod2-dimer\nFKBP65 supports LH2/PLOD2 dimer formation in the tested collagen-producing cell systems.\nPlain language: A helper protein helps assemble the collagen-modifying enzyme.\nCondition category: normal\norganism: Human\ntissue_or_cell_type: Not specified as a whole tissue; see experimental model.\nexperimental_model: Human collagen-producing fibroblasts and expression constructs; predominantly LH2B dimerization/interaction assays.\nlimitations: This is an experimentally scoped assembly mechanism; free lysine supplementation was not tested.\n[plod2-fkbp65-2016] Disentangling mechanisms involved in collagen pyridinoline cross-linking: The immunophilin FKBP65 is critical for dimerization of lysyl hydroxylase 2 (2016). https://pmc.ncbi.nlm.nih.gov/articles/PMC4932945/ DOI: 10.1073/pnas.1600074113","model_system":"Human collagen-producing fibroblasts and expression constructs; predominantly LH2B dimerization/interaction assays.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact quote from the accompanying curation document, not from publisher text. Original study references: [plod2-fkbp65-2016] Disentangling mechanisms involved in collagen pyridinoline cross-linking: The immunophilin FKBP65 is critical for dimerization of lysyl hydroxylase 2 (2016). https://pmc.ncbi.nlm.nih.gov/articles/PMC4932945/ DOI: 10.1073/pnas.1600074113","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7633bde7-dcc9-5086-91c6-a45eb96857f3","stable_key":"import-c3df3634-4c3a-5099-a5d9-e4f6344c1084","title":"L-Lysine: mechanism-first literature curation (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"93998d47c21525409ba82f1c82ededf2893dff15fbe61c7deffc175b0e298e97","revision_id":"3897e31f-6624-59e1-a053-8a81b7361632","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}