{"id":"cfb249cb-323a-53d1-bc4a-51ac5ca02037","stable_key":"97957230-601f-5dec-8524-0812be8fadbf:l-threonine-acsf3-methylmalonate","predicate":"when_deleted_increases","statement":"Acsf3 depletion increased labeled-threonine conversion to methylmalonic acid in mouse primary hepatocytes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"857d34c5-8b30-51bf-9086-20823f568bba","mechanism_event_label":"A downstream metabolic defect changed how threonine carbon accumulated.","subject":{"id":"a5610904-d941-5bd4-85bb-10417b4fe401","slug":"mouse-acsf3","display_name":"Mouse acyl-CoA synthetase family member 3 / Acsf3","entity_type_key":"protein"},"object":{"id":"73a46069-ac0f-59c3-bdd3-bb187b06c60d","slug":"mouse-threonine-methylmalonate-flux","display_name":"Mouse threonine-derived methylmalonate flux","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"857d34c5-8b30-51bf-9086-20823f568bba","stable_key":"97957230-601f-5dec-8524-0812be8fadbf:l-threonine-acsf3-methylmalonate-event","event_type":"observed_relationship","label":"A downstream metabolic defect changed how threonine carbon accumulated.","description":"Acsf3 depletion increased labeled-threonine conversion to methylmalonic acid in mouse primary hepatocytes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a5610904-d941-5bd4-85bb-10417b4fe401","slug":"mouse-acsf3","display_name":"Mouse acyl-CoA synthetase family member 3 / Acsf3","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"73a46069-ac0f-59c3-bdd3-bb187b06c60d","slug":"mouse-threonine-methylmalonate-flux","display_name":"Mouse threonine-derived methylmalonate flux","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"fcef4dc2-a7b6-5812-bc33-c8af3d83f4d0","slug":"l-threonine","display_name":"L-Threonine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"a2fda496-9308-54c5-b478-bc0b0c175570","slug":"methylmalonic-acid","display_name":"Methylmalonic acid","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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