{"id":"cf7e29db-0a5f-5d6a-9461-e798288195c0","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:apical-lysine-exchange","predicate":"transports","statement":"SLC7A9 associated with SLC3A1 mediates sodium-independent lysine exchange at the apical epithelial membrane.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"678da89e-4786-55dc-9722-6ef03a456b9e","mechanism_event_label":"This transporter admits lysine at the gut or kidney lumen-facing surface.","subject":{"id":"6b9c4d2d-f791-5a89-899f-9181506f458f","slug":"slc7a9-slc3a1-complex","display_name":"b0,+AT-rBAT complex","entity_type_key":"protein_complex"},"object":{"id":"5fff6664-209e-5c35-b88e-ac15c1c5fbc0","slug":"l-lysine","display_name":"L-Lysine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"678da89e-4786-55dc-9722-6ef03a456b9e","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:apical-lysine-exchange-event","event_type":"biochemical_relationship","label":"This transporter admits lysine at the gut or kidney lumen-facing surface.","description":"SLC7A9 associated with SLC3A1 mediates sodium-independent lysine exchange at the apical epithelial membrane.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"04b64edc-e647-5990-8cfd-2b97800b8b45","slug":"slc7a9","display_name":"b0,+AT / SLC7A9","entity_type_key":"protein"},"role":"transporter","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8607e7c9-d976-5f4a-b05d-adb3d3afce1f","slug":"slc3a1","display_name":"rBAT / SLC3A1","entity_type_key":"protein"},"role":"partner","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"5fff6664-209e-5c35-b88e-ac15c1c5fbc0","slug":"l-lysine","display_name":"L-Lysine","entity_type_key":"small_molecule"},"role":"transported_substrate","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"f707c753-cc8c-5f06-90be-bb3d73c60a18","slug":"leucine","display_name":"L-Leucine","entity_type_key":"small_molecule"},"role":"counter_substrate","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"6b9c4d2d-f791-5a89-899f-9181506f458f","slug":"slc7a9-slc3a1-complex","display_name":"b0,+AT-rBAT complex","entity_type_key":"protein_complex"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Human/mouse cloning, Xenopus transport and renal localization","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Leucine is an example exchange substrate, not the obligatory unique counter-substrate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Human and mouse proteins; Xenopus expression system","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"This transporter admits lysine at the gut or kidney lumen-facing surface.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[pfeiffer1999b0] Luminal Heterodimeric Amino Acid Transporter Defective in Cystinuria (1999). https://pmc.ncbi.nlm.nih.gov/articles/PMC25748/ DOI: 10.1091/mbc.10.12.4135","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Renal proximal-tubule brush border; intestinal apical context","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_effect","value_text":"depends","comparator":null,"unit":null,"notes":"The record names sodium-independent lysine exchange and not which way lysine moves in it.","entity":null},{"dimension":"transport_pool","value_text":"the enterocyte interior across the apical membrane","comparator":null,"unit":null,"notes":"The record names sodium-independent lysine exchange and not which way lysine moves in it.","entity":null}],"evidence":[{"id":"75c744a8-fbbd-5b85-9b71-ac1763023725","evidence_kind":"source_excerpt","locator":"Lines 24-32","start_line":24,"end_line":32,"excerpt":"### apical-lysine-exchange\nSLC7A9 associated with SLC3A1 mediates sodium-independent lysine exchange at the apical epithelial membrane.\nPlain language: This transporter admits lysine at the gut or kidney lumen-facing surface.\nCondition category: normal\norganism: Human and mouse proteins; Xenopus expression system\ntissue_or_cell_type: Renal proximal-tubule brush border; intestinal apical context\nexperimental_model: Human/mouse cloning, Xenopus transport and renal localization\nlimitations: Leucine is an example exchange substrate, not the obligatory unique counter-substrate.\n[pfeiffer1999b0] Luminal Heterodimeric Amino Acid Transporter Defective in Cystinuria (1999). https://pmc.ncbi.nlm.nih.gov/articles/PMC25748/ DOI: 10.1091/mbc.10.12.4135","model_system":"Human/mouse cloning, Xenopus transport and renal localization","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact quote from the accompanying curation document, not from publisher text. Original study references: [pfeiffer1999b0] Luminal Heterodimeric Amino Acid Transporter Defective in Cystinuria (1999). https://pmc.ncbi.nlm.nih.gov/articles/PMC25748/ DOI: 10.1091/mbc.10.12.4135","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7633bde7-dcc9-5086-91c6-a45eb96857f3","stable_key":"import-c3df3634-4c3a-5099-a5d9-e4f6344c1084","title":"L-Lysine: mechanism-first literature curation (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"93998d47c21525409ba82f1c82ededf2893dff15fbe61c7deffc175b0e298e97","revision_id":"3897e31f-6624-59e1-a053-8a81b7361632","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}