{"id":"cf7af258-69e8-52f0-8e0e-eed7aa243026","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-bio-pank4-pseudokinase","predicate":"lacks_detectable_kinase_activity_toward","statement":"Biochemical testing found that human PANK4 lacks pantothenate kinase activity, unlike active PANK1–3 enzymes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"cc3df57c-9d50-5ffd-8ef0-4ded33cefea5","mechanism_event_label":"The protein named PANK4 is not another working B5 kinase in humans.","subject":{"id":"19aa4674-db27-50f2-a232-44e427da4a5a","slug":"pank4","display_name":"Human pantothenate kinase 4 / PANK4","entity_type_key":"protein"},"object":{"id":"7bc62072-9517-5563-a3b8-8c5ab64a464b","slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"cc3df57c-9d50-5ffd-8ef0-4ded33cefea5","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-bio-pank4-pseudokinase-event","event_type":"biochemical_relationship","label":"The protein named PANK4 is not another working B5 kinase in humans.","description":"Biochemical testing found that human PANK4 lacks pantothenate kinase activity, unlike active PANK1–3 enzymes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"19aa4674-db27-50f2-a232-44e427da4a5a","slug":"pank4","display_name":"Human pantothenate kinase 4 / PANK4","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7bc62072-9517-5563-a3b8-8c5ab64a464b","slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant human PANK4 biochemistry with evolutionary sequence and mutagenesis comparisons","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Recombinant enzyme and catalytic-residue mutagenesis comparison; assay concentrations not extracted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This is evidence about an enzyme paralogue, not loss of all cellular pantothenate phosphorylation. Other species PANK4 enzymes differ.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The protein named PANK4 is not another working B5 kinase in humans.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b5-bio-pank4pseudo] Human pantothenate kinase 4 is a pseudo-pantothenate kinase. (2019). https://pubmed.ncbi.nlm.nih.gov/30927326/ DOI: 10.1002/pro.3611","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified recombinant protein; no intact tissue","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"02c10cdb-97e3-5a24-b632-570c6d83dcf2","evidence_kind":"source_excerpt","locator":"Lines 652-663","start_line":652,"end_line":663,"excerpt":"### b5-bio-pank4-pseudokinase\nBiochemical testing found that human PANK4 lacks pantothenate kinase activity, unlike active PANK1–3 enzymes.\nCondition category: normal\nnutrient_topic: Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The protein named PANK4 is not another working B5 kinase in humans.\norganism: Homo sapiens\ntissue_or_cell_type: Purified recombinant protein; no intact tissue\nexperimental_model: Recombinant human PANK4 biochemistry with evolutionary sequence and mutagenesis comparisons\nlimitations: This is evidence about an enzyme paralogue, not loss of all cellular pantothenate phosphorylation. Other species PANK4 enzymes differ.\nexposure: Recombinant enzyme and catalytic-residue mutagenesis comparison; assay concentrations not extracted.\ncross_nutrient: false\n[b5-bio-pank4pseudo] Human pantothenate kinase 4 is a pseudo-pantothenate kinase. (2019). https://pubmed.ncbi.nlm.nih.gov/30927326/ DOI: 10.1002/pro.3611","model_system":"Recombinant human PANK4 biochemistry with evolutionary sequence and mutagenesis comparisons","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b5-bio-pank4pseudo] Human pantothenate kinase 4 is a pseudo-pantothenate kinase. (2019). https://pubmed.ncbi.nlm.nih.gov/30927326/ DOI: 10.1002/pro.3611","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"0716b500-dd0b-5425-b9d0-d88bc9c09e86","stable_key":"import-f992b796-377d-53bf-a39b-7e5d41dbe194","title":"Pantothenic acid (vitamin B5): coenzyme A, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"b5a49f5a2373ca6064c9a4e014e052dad2f6f199a82bb09b26b82b831c36110b","revision_id":"78f3e793-f084-5ee2-8703-b661c4b650bf","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}