{"id":"cf73bd33-03d9-5ac3-a591-9fce652ac261","stable_key":"44737fa3-b335-53f4-a644-b9878d4416ac:creatine-gaa-complex-ii","predicate":"inhibited","statement":"Guanidinoacetate inhibited complex II activity in the in-vitro striatal assays; complex II–III activity was inhibited after intrastriatal exposure.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"194faeaf-bea2-5b73-9291-c20f7ef9010e","mechanism_event_label":"The accumulated precursor can affect mitochondrial enzyme measurements in this model.","subject":{"id":"84053a83-8fcc-5068-8df9-58f7e97fad76","slug":"guanidinoacetate","display_name":"Guanidinoacetate","entity_type_key":"small_molecule"},"object":{"id":"a6bd53b7-7cab-57c1-b59d-f8f5bafae3d9","slug":"striatal-complex-ii-activity","display_name":"Rat striatal respiratory complex II activity","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"194faeaf-bea2-5b73-9291-c20f7ef9010e","stable_key":"44737fa3-b335-53f4-a644-b9878d4416ac:creatine-gaa-complex-ii-event","event_type":"biochemical_relationship","label":"The accumulated precursor can affect mitochondrial enzyme measurements in this model.","description":"Guanidinoacetate inhibited complex II activity in the in-vitro striatal assays; complex II–III activity was inhibited after intrastriatal exposure.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"84053a83-8fcc-5068-8df9-58f7e97fad76","slug":"guanidinoacetate","display_name":"Guanidinoacetate","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"a6bd53b7-7cab-57c1-b59d-f8f5bafae3d9","slug":"striatal-complex-ii-activity","display_name":"Rat striatal respiratory complex II activity","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/creatine-research/17407807.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"6174f6169ebf6ca7e61bc61642e38841a9089aecdd278ad8fb2f2221cf847c35\", \"start_char\": 0, \"end_char\": 1929, \"text_sha256\": \"6174f6169ebf6ca7e61bc61642e38841a9089aecdd278ad8fb2f2221cf847c35\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Acute guanidinoacetate exposure with biochemical assays and antioxidant pretreatment","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Intrastriatal guanidinoacetate and in-vitro assays; taurine or combined vitamins E plus C pretreatment","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This scenario models a consequence relevant to a GAMT bottleneck, but the experiment administered guanidinoacetate rather than deleting GAMT. 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No human treatment inference.\nexposure: Intrastriatal guanidinoacetate and in-vitro assays; taurine or combined vitamins E plus C pretreatment\nevidence_span: {\"source_cache\": \"artifacts/creatine-research/17407807.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"6174f6169ebf6ca7e61bc61642e38841a9089aecdd278ad8fb2f2221cf847c35\", \"start_char\": 0, \"end_char\": 1929, \"text_sha256\": \"6174f6169ebf6ca7e61bc61642e38841a9089aecdd278ad8fb2f2221cf847c35\"}\n[creatine-p17407807] Evidence that the inhibitory effects of guanidinoacetate on the activities of the respiratory chain, Na+,K+-ATPase and creatine kinase can be differentially prevented by taurine and vitamins E and C administration in rat striatum in vivo. 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