{"id":"cf57b127-816f-54dd-874f-0c9dfb6a4e2c","stable_key":"3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd:silica-osteoclast-differentiation","predicate":"reduces","statement":"The 75%-OSA silicon preparation reduced RANKL-stimulated TRAP-positive multinucleated human osteoclasts.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"c97c4b3c-c18c-5ede-80e4-3a8b4072b386","mechanism_event_label":"Fewer bone-resorbing cells formed under these culture conditions.","subject":{"id":"1ff7d527-b221-5c48-b48d-a015f7aebe6e","slug":"osa-enriched-silicon-osteoclast-preparation","display_name":"Silicon preparation containing 75% orthosilicic acid","entity_type_key":"chemical_species"},"object":{"id":"6db3cbef-401d-5ef3-ab6f-e6949aab7b40","slug":"human-osa-osteoclast-differentiation","display_name":"Human osteoclast differentiation under silicon exposure","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"c97c4b3c-c18c-5ede-80e4-3a8b4072b386","stable_key":"3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd:silica-osteoclast-differentiation-event","event_type":"observed_relationship","label":"Fewer bone-resorbing cells formed under these culture conditions.","description":"The 75%-OSA silicon preparation reduced RANKL-stimulated TRAP-positive multinucleated human osteoclasts.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"1ff7d527-b221-5c48-b48d-a015f7aebe6e","slug":"osa-enriched-silicon-osteoclast-preparation","display_name":"Silicon preparation containing 75% orthosilicic acid","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6db3cbef-401d-5ef3-ab6f-e6949aab7b40","slug":"human-osa-osteoclast-differentiation","display_name":"Human osteoclast differentiation under silicon exposure","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"11b1cdb5-1b10-586b-9917-b4da18ac072f","slug":"silica","display_name":"Silica and soluble silicon","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human CD14+ monocytes, M-CSF and recombinant mouse RANKL; 50 micrograms Si/mL.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"About 1.8 mM total Si, not a normal plasma exposure or chemically pure OSA.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Silica collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"silica","display_name":"Silica and soluble silicon","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"Fewer bone-resorbing cells formed under these culture conditions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Orthosilicic acid inhibits human osteoclast differentiation and bone resorption. · 2024 · https://pubmed.ncbi.nlm.nih.gov/39405281/ · DOI 10.1371/journal.pone.0312169","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"2f9a9aec-fbb6-5186-97cb-88071e918cf4","evidence_kind":"source_excerpt","locator":"Lines 216-222","start_line":216,"end_line":222,"excerpt":"## silica-osteoclast-differentiation\nFewer bone-resorbing cells formed under these culture conditions.\nThe 75%-OSA silicon preparation reduced RANKL-stimulated TRAP-positive multinucleated human osteoclasts.\nModel: Human CD14+ monocytes, M-CSF and recombinant mouse RANKL; 50 micrograms Si/mL.\nLimitations: About 1.8 mM total Si, not a normal plasma exposure or chemically pure OSA.\nEvidence access: Primary full text\nOrthosilicic acid inhibits human osteoclast differentiation and bone resorption. · 2024 · https://pubmed.ncbi.nlm.nih.gov/39405281/ · DOI 10.1371/journal.pone.0312169","model_system":"Human CD14+ monocytes, M-CSF and recombinant mouse RANKL; 50 micrograms Si/mL.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dc4b461e-dd46-57ab-8213-8aeb7ff9e8d0","stable_key":"import-3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd","title":"Silica: soluble silicon, cellular transport and particle-specific mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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