{"id":"cf579ab1-4a4a-5d1f-814d-e1128e4b94eb","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-t-cell-transport","predicate":"increases_expression","statement":"Activation increased SNAT1 transcript and protein in mouse T cells alongside their increased requirement for extracellular alanine.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"7794180e-da36-5e19-9704-7204020edff4","mechanism_event_label":"Activated cells increase machinery for bringing alanine inside.","subject":{"id":"fc5fb410-f62b-57e1-9eae-b94d1532db04","slug":"mouse-t-cell-activation-growth","display_name":"Growth and proliferation during mouse T-cell activation","entity_type_key":"cellular_process"},"object":{"id":"497a024e-35c1-57d1-8c79-a58427de6446","slug":"mouse-slc38a1","display_name":"Mouse sodium-coupled neutral amino acid transporter 1 / Slc38a1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"7794180e-da36-5e19-9704-7204020edff4","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-t-cell-transport-event","event_type":"observed_relationship","label":"Activated cells increase machinery for bringing alanine inside.","description":"Activation increased SNAT1 transcript and protein in mouse T cells alongside their increased requirement for extracellular alanine.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"fc5fb410-f62b-57e1-9eae-b94d1532db04","slug":"mouse-t-cell-activation-growth","display_name":"Growth and proliferation during mouse T-cell activation","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"497a024e-35c1-57d1-8c79-a58427de6446","slug":"mouse-slc38a1","display_name":"Mouse sodium-coupled neutral amino acid transporter 1 / Slc38a1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Primary mouse naive CD4/CD8 cells activated ex vivo; dialyzed-serum medium with alanine add-back.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Expression was measured; this experiment did not genetically prove that SNAT1 alone accounts for alanine uptake.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Alanine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Activated cells increase machinery for bringing alanine inside.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"T Cell Activation Depends on Extracellular Alanine. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31533027/ · DOI 10.1016/j.celrep.2019.08.034","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"1ad98d3f-f2c2-5522-98ae-7b9ac3dab1e9","evidence_kind":"source_excerpt","locator":"Lines 88-94","start_line":88,"end_line":94,"excerpt":"## alanine-t-cell-transport\nActivated cells increase machinery for bringing alanine inside.\nActivation increased SNAT1 transcript and protein in mouse T cells alongside their increased requirement for extracellular alanine.\nModel: Primary mouse naive CD4/CD8 cells activated ex vivo; dialyzed-serum medium with alanine add-back.\nLimitations: Expression was measured; this experiment did not genetically prove that SNAT1 alone accounts for alanine uptake.\nEvidence access: Primary full text\nT Cell Activation Depends on Extracellular Alanine. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31533027/ · DOI 10.1016/j.celrep.2019.08.034","model_system":"Primary mouse naive CD4/CD8 cells activated ex vivo; dialyzed-serum medium with alanine add-back.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"01c5554c-a8ba-5c86-9b01-a0fa1a886cdb","stable_key":"import-10aa417f-4b03-599f-a453-4aa09edeb27c","title":"L-Alanine: carbon, nitrogen, protein synthesis and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"8ce7dd76c88b2e25fa23d93f9655a244aadb9d90f55ec8b18ecf0fd4625a61d2","revision_id":"4ed32258-0213-599a-a272-6f8f62280773","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}