{"id":"cf4044cb-2f5a-5957-86ab-5967a40064da","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-e-no-g-actin-binding","predicate":"does_not_bind_in_tested_assay","statement":"The cucurbitacin E actin study reported binding to filamentous actin but not globular actin.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"d02de078-6323-5366-96fe-719781370e2c","mechanism_event_label":"The physical state of the protein changes the result.","subject":{"id":"d5631638-0ee3-537f-b7cc-cbd4958395d3","slug":"cucurbitacin-e","display_name":"Cucurbitacin E","entity_type_key":"small_molecule"},"object":{"id":"7633cd49-57aa-5817-b6e9-f26a4334ca42","slug":"purified-cucurbitacin-g-actin","display_name":"Purified G-actin in cucurbitacin assays, species as reported","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d02de078-6323-5366-96fe-719781370e2c","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-e-no-g-actin-binding-event","event_type":"observed_relationship","label":"The physical state of the protein changes the result.","description":"The cucurbitacin E actin study reported binding to filamentous actin but not globular actin.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"d5631638-0ee3-537f-b7cc-cbd4958395d3","slug":"cucurbitacin-e","display_name":"Cucurbitacin E","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7633cd49-57aa-5817-b6e9-f26a4334ca42","slug":"purified-cucurbitacin-g-actin","display_name":"Purified G-actin in cucurbitacin assays, species as reported","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Same purified-actin experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Member-specific assay result; cannot be transferred to cucurbitacin I.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Cucurbitacins collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"cucurbitacin","display_name":"Cucurbitacins","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"The physical state of the protein changes the result.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The natural product cucurbitacin E inhibits depolymerization of actin filaments. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22724897/ · DOI 10.1021/cb300254s","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"2a0fda51-c109-5986-ad2c-ae9004629ff5","evidence_kind":"source_excerpt","locator":"Lines 60-66","start_line":60,"end_line":66,"excerpt":"## cucurbitacin-e-no-g-actin-binding\nThe physical state of the protein changes the result.\nThe cucurbitacin E actin study reported binding to filamentous actin but not globular actin.\nModel: Same purified-actin experiments.\nLimitations: Member-specific assay result; cannot be transferred to cucurbitacin I.\nEvidence access: Primary abstract\nThe natural product cucurbitacin E inhibits depolymerization of actin filaments. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22724897/ · DOI 10.1021/cb300254s","model_system":"Same purified-actin experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"77324310-00ce-5d28-9fc7-595fddae86b9","stable_key":"import-2921131d-0ba0-51d3-8f59-8c4be3b9ac08","title":"Cucurbitacins: thiol chemistry, cytoskeleton, metabolic dependencies and signaling (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"5c23ec61b9cf3d2966fb081d8617e1e1f7c522e86419b8ba2dcae09590121123","revision_id":"74251a06-3ba5-506c-b428-700eca0fb432","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}