{"id":"cf339d7f-1db6-5241-90fb-400089a8d641","stable_key":"ec987f3e-0b3c-55cc-b304-c703a738e35d:egcg-pi3k","predicate":"inhibits","statement":"EGCG showed ATP-competitive inhibition with Ki 380 nanomolar in the reported enzyme assay.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"205be748-fa46-5be8-8109-e9cff2bd5d3c","mechanism_event_label":"EGCG competed with the enzyme’s energy substrate.","subject":{"id":"22e1b8eb-f35f-5afa-be20-b0534df9f6be","slug":"egcg","display_name":"Epigallocatechin-3-gallate (EGCG)","entity_type_key":"small_molecule"},"object":{"id":"359979da-c958-50bf-b2f7-9f15e177fa5d","slug":"pi3k-family","display_name":"Phosphoinositide 3-kinase family","entity_type_key":"protein_family"},"evidence_count":1,"mechanism_event":{"id":"205be748-fa46-5be8-8109-e9cff2bd5d3c","stable_key":"ec987f3e-0b3c-55cc-b304-c703a738e35d:egcg-pi3k-event","event_type":"observed_relationship","label":"EGCG competed with the enzyme’s energy substrate.","description":"EGCG showed ATP-competitive inhibition with Ki 380 nanomolar in the reported enzyme assay.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"22e1b8eb-f35f-5afa-be20-b0534df9f6be","slug":"egcg","display_name":"Epigallocatechin-3-gallate (EGCG)","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"359979da-c958-50bf-b2f7-9f15e177fa5d","slug":"pi3k-family","display_name":"Phosphoinositide 3-kinase family","entity_type_key":"protein_family"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Biochemical kinase assays plus MDA-MB-231 and A549 cell experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"PI3K isoform is not assigned from abstract; docking is a model, not a solved ligand structure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"EGCG collection; comparator and shared-pathway records retain their actual intervention.","comparator":null,"unit":null,"notes":"","entity":{"slug":"egcg","display_name":"Epigallocatechin-3-gallate (EGCG)","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"EGCG competed with the enzyme’s energy substrate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Epigallocatechin gallate (EGCG), a major component of green tea, is a dual phosphoinositide-3-kinase/mTOR inhibitor. · 2011 · https://pubmed.ncbi.nlm.nih.gov/21300025/ · DOI 10.1016/j.bbrc.2011.02.010","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"e848d3a4-72e3-5bb1-8779-607202285781","evidence_kind":"source_excerpt","locator":"Lines 212-218","start_line":212,"end_line":218,"excerpt":"## egcg-pi3k\nEGCG competed with the enzyme’s energy substrate.\nEGCG showed ATP-competitive inhibition with Ki 380 nanomolar in the reported enzyme assay.\nModel: Biochemical kinase assays plus MDA-MB-231 and A549 cell experiments.\nLimitations: PI3K isoform is not assigned from abstract; docking is a model, not a solved ligand structure.\nEvidence access: primary abstract.\nEpigallocatechin gallate (EGCG), a major component of green tea, is a dual phosphoinositide-3-kinase/mTOR inhibitor. · 2011 · https://pubmed.ncbi.nlm.nih.gov/21300025/ · DOI 10.1016/j.bbrc.2011.02.010","model_system":"Biochemical kinase assays plus MDA-MB-231 and A549 cell experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Primary-abstract paraphrase; no full-text methods verification claimed.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1f6d09e8-3aae-5c41-9ea7-15d0d1cab72d","stable_key":"import-ec987f3e-0b3c-55cc-b304-c703a738e35d","title":"EGCG: receptor signaling, metabolism, nutrient interactions and discovery questions (2026-09-18)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"b101c8d4d96ee03ff97eb1922c7bb0f96943295af473fdc3ef98b0dbd62fe8c7","revision_id":"e19bc5b2-ee3c-5f67-b5c5-9dc3b7d5f850","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}