{"id":"cf2f7f2b-bcdf-5405-920b-30362302f75d","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-tfeb-proline","predicate":"mutation_partly_bypasses","statement":"Replacing the TFEB PPP motif with AAA partially rescued TFEB expression during GC7 treatment.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"ed6da466-3936-5382-b2c5-4ab5f77073f9","mechanism_event_label":"A sequence within the protein helps explain the dependency.","subject":{"id":"462b0422-378e-528e-a0d4-a029a9778c2c","slug":"mouse-tfeb-triproline-motif","display_name":"Mouse TFEB triproline-containing translation motif","entity_type_key":"cellular_process"},"object":{"id":"bdd7a685-e772-55c0-a183-bc0bdccb61cc","slug":"mouse-tfeb","display_name":"Mouse transcription factor EB / Tfeb","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"ed6da466-3936-5382-b2c5-4ab5f77073f9","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-tfeb-proline-event","event_type":"observed_relationship","label":"A sequence within the protein helps explain the dependency.","description":"Replacing the TFEB PPP motif with AAA partially rescued TFEB expression during GC7 treatment.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"462b0422-378e-528e-a0d4-a029a9778c2c","slug":"mouse-tfeb-triproline-motif","display_name":"Mouse TFEB triproline-containing translation motif","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"bdd7a685-e772-55c0-a183-bc0bdccb61cc","slug":"mouse-tfeb","display_name":"Mouse transcription factor EB / Tfeb","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7014993c-468f-5c6b-ab04-80c87f9dfd9f","slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Murine TFEB constructs in NIH 3T3 cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Partial rescue; free proline supplementation was not tested.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Spermidine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A sequence within the protein helps explain the dependency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Polyamines Control eIF5A Hypusination, TFEB Translation, and Autophagy to Reverse B Cell Senescence. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31474573/ · DOI 10.1016/j.molcel.2019.08.005","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"696eb77a-bdfd-5d63-8396-dcff1166a7c9","evidence_kind":"source_excerpt","locator":"Lines 222-228","start_line":222,"end_line":228,"excerpt":"## spermidine-tfeb-proline\nA sequence within the protein helps explain the dependency.\nReplacing the TFEB PPP motif with AAA partially rescued TFEB expression during GC7 treatment.\nModel: Murine TFEB constructs in NIH 3T3 cells.\nLimitations: Partial rescue; free proline supplementation was not tested.\nEvidence access: Primary full text\nPolyamines Control eIF5A Hypusination, TFEB Translation, and Autophagy to Reverse B Cell Senescence. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31474573/ · DOI 10.1016/j.molcel.2019.08.005","model_system":"Murine TFEB constructs in NIH 3T3 cells.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4348f3f8-0180-58a3-b61c-55d03bb322d2","stable_key":"import-88eb7407-f147-558b-889b-0c89c4e0aa4a","title":"Spermidine: biosynthesis, hypusination, transport and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"8eec8784bc2bd0384cfd53c6603c4ced8e53c227d9ab29ed85594c43e34c68ef","revision_id":"7c421a18-4526-59f6-b84e-4be533bc35ac","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}