{"id":"cf0b25cf-573f-5f6c-a3fb-4bfd215652b3","stable_key":"6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7:stevia-ugt1a3","predicate":"conjugates","statement":"UGT1A3 contributed alongside UGT2B7 to steviol glucuronidation at high substrate concentration.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"86e0408c-3b28-5d3e-be21-f818c7ca4a7b","mechanism_event_label":"The participating enzyme mix changes with substrate concentration.","subject":{"id":"761185ae-f947-5d07-942b-6320d3d6e415","slug":"ugt1a3","display_name":"Human UDP-glucuronosyltransferase 1A3 / UGT1A3","entity_type_key":"protein"},"object":{"id":"8ba32fe6-183f-5cd5-b386-02e6440e78a0","slug":"steviol","display_name":"Steviol","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"86e0408c-3b28-5d3e-be21-f818c7ca4a7b","stable_key":"6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7:stevia-ugt1a3-event","event_type":"observed_relationship","label":"The participating enzyme mix changes with substrate concentration.","description":"UGT1A3 contributed alongside UGT2B7 to steviol glucuronidation at high substrate concentration.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"761185ae-f947-5d07-942b-6320d3d6e415","slug":"ugt1a3","display_name":"Human UDP-glucuronosyltransferase 1A3 / UGT1A3","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8ba32fe6-183f-5cd5-b386-02e6440e78a0","slug":"steviol","display_name":"Steviol","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c78086c3-7657-589b-b353-1691df2d9f8f","slug":"stevia","display_name":"Stevia","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"f008b74d-164b-5663-a72e-04a48fe7a8fb","slug":"steviol-glucuronide","display_name":"Steviol glucuronide","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"fea4f3f0-7c05-59a2-9332-445b9d4f8fb0","slug":"udp-glucuronic-acid","display_name":"UDP-glucuronic acid","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant human enzyme screening and microsomal kinetics.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"High versus low assay concentration must not be converted into an unmeasured dietary threshold.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Stevia collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"stevia","display_name":"Stevia","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The participating enzyme mix changes with substrate concentration.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Steviol glucuronidation and its potential interaction with UDP-glucuronosyltransferase 2B7 substrates. · 2014 · https://pubmed.ncbi.nlm.nih.gov/24296138/ · DOI 10.1016/j.fct.2013.11.028","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"434e48db-dc77-5947-854d-0271368af8e5","evidence_kind":"source_excerpt","locator":"Lines 58-64","start_line":58,"end_line":64,"excerpt":"## stevia-ugt1a3\nThe participating enzyme mix changes with substrate concentration.\nUGT1A3 contributed alongside UGT2B7 to steviol glucuronidation at high substrate concentration.\nModel: Recombinant human enzyme screening and microsomal kinetics.\nLimitations: High versus low assay concentration must not be converted into an unmeasured dietary threshold.\nEvidence access: Primary abstract\nSteviol glucuronidation and its potential interaction with UDP-glucuronosyltransferase 2B7 substrates. · 2014 · https://pubmed.ncbi.nlm.nih.gov/24296138/ · DOI 10.1016/j.fct.2013.11.028","model_system":"Recombinant human enzyme screening and microsomal kinetics.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; 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