{"id":"ceb6609c-1f7b-5787-8e64-378b32bed246","stable_key":"18100170-a014-51c3-adeb-9dab4162b8cc:pituitary-gh-up","predicate":"increases_in_recorded_experiment","statement":"Higher-concentration thymulin stimulated GH release in dispersed rat pituitary preparations; responses depended on age and concentration.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"55a24719-ede4-5db9-88b0-945a680d9623","mechanism_event_label":"Higher-concentration thymulin stimulated GH release in dispersed rat pituitary preparations; responses depended on age and concentration.","subject":{"id":"23819462-9bdf-5856-beac-68b3dca9cf85","slug":"thymulin","display_name":"Thymulin","entity_type_key":"peptide"},"object":{"id":"93f426ba-6772-5feb-974b-9f045ca73979","slug":"rat-pituitary-gh-release","display_name":"Rat anterior-pituitary growth-hormone release","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"55a24719-ede4-5db9-88b0-945a680d9623","stable_key":"18100170-a014-51c3-adeb-9dab4162b8cc:pituitary-gh-up-event","event_type":"experimental_observation","label":"Higher-concentration thymulin stimulated GH release in dispersed rat pituitary preparations; responses depended on age and concentration.","description":"In a different dispersed-rat-pituitary study using higher concentrations, thymulin stimulated **GH** release; responses depended on age and concentration. EGTA blocked activity, and cAMP enhancers potentiated it. These interventions support calcium-sensitive signaling, not a requirement for calcium bound directly to thymulin. The earlier GH null remains part of the evidence. [1999 GH study](https://pubmed.ncbi.nlm.nih.gov/9892847/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"23819462-9bdf-5856-beac-68b3dca9cf85","slug":"thymulin","display_name":"Thymulin","entity_type_key":"peptide"},"role":"tested factor","stoichiometry":null,"state_label":"Thymulin","sequence_order":0,"notes":""},{"entity":{"id":"93f426ba-6772-5feb-974b-9f045ca73979","slug":"rat-pituitary-gh-release","display_name":"Rat anterior-pituitary growth-hormone release","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"increase","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary bibliographic record and abstract checked; full methods/figures not comprehensively reviewed.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Thymulin\", \"comparator\": \"Matched cell preparation\", \"endpoint\": \"Higher-concentration thymulin stimulated GH release in dispersed rat pituitary preparations; responses depended on age and concentration.\", \"effect_direction\": \"increase\", \"combination\": \"single\", \"conditions\": []}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Dispersed rat pituitary cells; higher concentration than earlier tissue study; no universal GH sign.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Interpret only within the recorded preparation, exposure and comparator. The complete source passage retains qualifications; unspecified doses/timing have not been extracted here. No clinical efficacy, nutrient deficiency or unique molecular mediation is inferred.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Higher-concentration thymulin stimulated GH release in dispersed rat pituitary preparations; responses depended on age and concentration.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Growth hormone-releasing activity of thymulin on pituitary somatotropes is age dependent. | 1999 | DOI 10.1159/000054399 | PMID 9892847 | https://pubmed.ncbi.nlm.nih.gov/9892847/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 106-106; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"78336eea-0474-5087-8146-25c5d2027bff","evidence_kind":"source_excerpt","locator":"Lines 106-106","start_line":106,"end_line":106,"excerpt":"In a different dispersed-rat-pituitary study using higher concentrations, thymulin stimulated **GH** release; responses depended on age and concentration. EGTA blocked activity, and cAMP enhancers potentiated it. These interventions support calcium-sensitive signaling, not a requirement for calcium bound directly to thymulin. The earlier GH null remains part of the evidence. [1999 GH study](https://pubmed.ncbi.nlm.nih.gov/9892847/)","model_system":"Dispersed rat pituitary cells; higher concentration than earlier tissue study; no universal GH sign.","directness":"reported_statement","verification_status":"source_recorded","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. Not a verbatim quotation from a primary paper.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e1871d49-9c6b-547b-a1bb-6e2b9270ca89","stable_key":"import-18100170-a014-51c3-adeb-9dab4162b8cc","title":"Thymulin: detailed mechanisms of action (reviewed 3 October 2026)","document_type":"imported_text","citation_label":"Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication.","file_path":"","sha256":"6e8ed0d7a5e23fc822f2ab418e0c4fb2411c01fcaa330039e8d7d93218cacc88","revision_id":"8c0e3449-9bbd-57c0-bb5e-de83f044f2ac","review_status":"source_recorded","notes":""}}],"relations":[{"id":"ecb5203b-80d7-5dcf-a26f-26ddefaf6d47","relation_type":"limits_scope_of","notes":"The GH null at picomolar exposure in isolated tissue limits a universal GH-stimulation claim; different preparation/concentration is retained rather than called a corrected error.","source_claim_id":"2527a1a1-1080-5187-93f6-5a57d146437e","source_claim_key":"18100170-a014-51c3-adeb-9dab4162b8cc:pituitary-gh-null","source_statement":"GH did not increase in the picomolar zinc-thymulin isolated rat anterior-pituitary experiment.","target_claim_id":"ceb6609c-1f7b-5787-8e64-378b32bed246","target_claim_key":"18100170-a014-51c3-adeb-9dab4162b8cc:pituitary-gh-up","target_statement":"Higher-concentration thymulin stimulated GH release in dispersed rat pituitary preparations; responses depended on age and concentration."}],"conflicts":[],"corrections":[],"research":null}