{"id":"ceac4a01-a7a4-5c54-b281-07aeaf36d78a","stable_key":"fd7915ff-856d-56c2-a118-4989693a7644:gingerols-8-mlc","predicate":"reduces","statement":"Adding 8-gingerol reduced myosin light-chain phosphorylation in human airway smooth muscle cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"c1589b9a-3743-52b9-ba94-38322425f5d5","mechanism_event_label":"Adding 8-gingerol reduced myosin light-chain phosphorylation in human airway smooth muscle cells.","subject":{"id":"d15f1089-e355-5f5e-8c76-5021ade1dc75","slug":"8-gingerol","display_name":"8-Gingerol","entity_type_key":"small_molecule"},"object":{"id":"9d88b3d9-e64d-50df-bd44-f63c8f1649ef","slug":"human-airway-myosin-light-chain-phosphorylation","display_name":"Myosin light-chain phosphorylation in human airway smooth muscle cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"c1589b9a-3743-52b9-ba94-38322425f5d5","stable_key":"fd7915ff-856d-56c2-a118-4989693a7644:gingerols-8-mlc-event","event_type":"observed_relationship","label":"Adding 8-gingerol reduced myosin light-chain phosphorylation in human airway smooth muscle cells.","description":"Adding 8-gingerol reduced myosin light-chain phosphorylation in human airway smooth muscle cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"d15f1089-e355-5f5e-8c76-5021ade1dc75","slug":"8-gingerol","display_name":"8-Gingerol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9d88b3d9-e64d-50df-bd44-f63c8f1649ef","slug":"human-airway-myosin-light-chain-phosphorylation","display_name":"Myosin light-chain phosphorylation in human airway smooth muscle cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"8-Gingerol; study tissue exposure 100 micromolar, cell-assay details not resolved in accessed abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Not specified in accessed abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation, not universally established human effects","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Primary human airway smooth muscle cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Reduced phosphorylation does not establish direct binding to myosin or a clinical bronchodilator response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Gingerols collection; each molecular form and experimental preparation remains explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"gingerols","display_name":"Gingerols","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Primary human airway smooth muscle cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Adding 8-gingerol reduced myosin light-chain phosphorylation in human airway smooth muscle cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Active components of ginger potentiate β-agonist-induced relaxation of airway smooth muscle by modulating cytoskeletal regulatory proteins. (2014). https://pubmed.ncbi.nlm.nih.gov/23962082/ DOI: 10.1165/rcmb.2013-0133OC","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro addition","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Cultured airway smooth muscle","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ef078b35-bc75-5a8e-9dbd-6db2d6d87364","evidence_kind":"source_excerpt","locator":"Lines 250-259","start_line":250,"end_line":259,"excerpt":"## gingerols-8-mlc\nAdding 8-gingerol reduced myosin light-chain phosphorylation in human airway smooth muscle cells.\nModel/species: Primary human airway smooth muscle cells\nTissue: Cultured airway smooth muscle\nExposure: 8-Gingerol; study tissue exposure 100 micromolar, cell-assay details not resolved in accessed abstract\nRoute: In vitro addition\nDuration: Not specified in accessed abstract\nLimits: Reduced phosphorylation does not establish direct binding to myosin or a clinical bronchodilator response.\nPrimary reference: Active components of ginger potentiate β-agonist-induced relaxation of airway smooth muscle by modulating cytoskeletal regulatory proteins. (2014). https://pubmed.ncbi.nlm.nih.gov/23962082/ DOI: 10.1165/rcmb.2013-0133OC\nAccess: Primary PubMed abstract.","model_system":"Primary human airway smooth muscle cells","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact archived synthesis span with its primary citation; original paraphrase, not a claimed publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1107d60b-8e2d-55d7-8f5d-5612c49aa7dc","stable_key":"import-fd7915ff-856d-56c2-a118-4989693a7644","title":"Gingerols: mechanisms, molecular forms and cross-actor connections (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted curation of thirteen primary research papers; study-specific PubMed/DOI links and limitations retained. 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