{"id":"ce80aa9b-7071-5590-b2b7-65122b331296","stable_key":"research:selenoi-synthesizes-diacyl-pe","predicate":"synthesizes","statement":"SELENOI transfers phosphoethanolamine from CDP-ethanolamine to diacylglycerol, producing diacyl PE and CMP.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"literature_reviewed:direct_experimental","direction":"neutral","is_public":true,"mechanism_event_id":"e904624d-8dbb-5d7e-a9ed-566664e825a9","mechanism_event_label":"SELENOI builds phosphatidylethanolamine from an activated headgroup and a lipid backbone.","subject":{"id":"8334e065-581a-5500-b664-dbe129850043","slug":"selenoi","display_name":"SELENOI","entity_type_key":"protein"},"object":{"id":"be4a52f4-f31c-59b7-b0da-4178fbb069c8","slug":"phosphatidylethanolamine","display_name":"Phosphatidylethanolamine","entity_type_key":"lipid"},"evidence_count":1,"mechanism_event":{"id":"e904624d-8dbb-5d7e-a9ed-566664e825a9","stable_key":"research:selenoi-synthesizes-diacyl-pe","event_type":"phosphotransfer","label":"SELENOI builds phosphatidylethanolamine from an activated headgroup and a lipid backbone.","description":"SELENOI transfers phosphoethanolamine from CDP-ethanolamine to diacylglycerol, producing diacyl PE and CMP.","status":"active","compartment":null,"participants":[{"entity":{"id":"8334e065-581a-5500-b664-dbe129850043","slug":"selenoi","display_name":"SELENOI","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"53b36ed3-4463-5621-ac99-a4e5e360f05e","slug":"cdp-ethanolamine","display_name":"CDP-ethanolamine","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"13ba4537-bd0f-5817-9aae-aa255edcb5d6","slug":"diacylglycerol","display_name":"DAG","entity_type_key":"lipid"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"be4a52f4-f31c-59b7-b0da-4178fbb069c8","slug":"phosphatidylethanolamine","display_name":"Phosphatidylethanolamine","entity_type_key":"lipid"},"role":"product","stoichiometry":null,"state_label":"diacyl form","sequence_order":3,"notes":""},{"entity":{"id":"729ece0d-0058-5133-a6b7-c68c304be2eb","slug":"cmp","display_name":"CMP","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"cell_type","value_text":"patient fibroblasts and HeLa","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Lipid synthesis and genetic studies","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Other enzymes can contribute to diacyl PE.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"human","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"3f7e8201-be91-5b87-9a5e-5f5045ac73fa","evidence_kind":"curated_literature_summary","locator":"lines 726-736","start_line":726,"end_line":736,"excerpt":"## selenoi-synthesizes-diacyl-pe\n\nSELENOI builds phosphatidylethanolamine from an activated headgroup and a lipid backbone.\n\nSELENOI transfers phosphoethanolamine from CDP-ethanolamine to diacylglycerol, producing diacyl PE and CMP.\n\nOrganism: human\nCell type: patient fibroblasts and HeLa\nExperimental model: Lipid synthesis and genetic studies\nLimitations: Other enzymes can contribute to diacyl PE.\nPrimary reference: [EPT1 (selenoprotein I) is critical for the neural development and maintenance of plasmalogen in humans](https://pmc.ncbi.nlm.nih.gov/articles/PMC5983406/)","model_system":"Lipid synthesis and genetic studies","directness":"author_interpretation","verification_status":"secondary_verified","notes":"Curated summary; inspect the linked primary papers for original methods and results.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f892f13-06ea-5199-a33c-a703f35c80ae","stable_key":"selenium-research-2026-09-17","title":"Selenium: literature corrections and mechanism additions","document_type":"curated_literature_review","citation_label":"Metabolic Ledger literature curation, 17 September 2026; primary papers linked individually","file_path":"","sha256":"0b818b10c1c7120e5caf7f4d4019d7bd025d745692e424f515d3ef903c9ab7f3","revision_id":"80984e03-5f0f-5877-8094-afef7637444e","review_status":"secondary_verified","notes":"Secondary curated summaries of primary experiments, with explicit models and limitations. Not archived primary full text."}}],"relations":[],"conflicts":[],"corrections":[],"research":{"topic":"lipid-biosynthesis","plain_language":"SELENOI builds phosphatidylethanolamine from an activated headgroup and a lipid backbone.","evidence_scope":"direct_experimental","papers":[{"key":"horibata-2018-ept1-lipids","title":"EPT1 (selenoprotein I) is critical for the neural development and maintenance of plasmalogen in humans","url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC5983406/","doi":"10.1194/jlr.P081620","year":2018,"model":"Human patient fibroblasts, HeLa gene disruption and lipid assays","summary":"Defines SELENOI-dependent phosphatidylethanolamine production and consequences for ether lipids."}]}}