{"id":"ce5c1ce1-2e4d-5aa8-ab5c-149e46535f11","stable_key":"488ea171-36b8-5ef6-b5d3-98563980fdbf:hbot-pressure-alone-ineffective","predicate":"preserves_measured_pool","statement":"Exposing rats to 3 ATA pressure with 0.21 ATA oxygen after poisoning had no significant effects, and 1 ATA oxygen did not significantly inhibit xanthine oxidase formation or lipid peroxidation.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"50e97bbc-3a04-57d4-babb-9e688cbe0cd2","mechanism_event_label":"Pressure by itself did nothing; it is the oxygen at pressure that matters.","subject":{"id":"31bd23dc-1798-52f9-b1fe-d364807d184c","slug":"chamber-pressure-alone","display_name":"Raised chamber pressure without raised oxygen fraction","entity_type_key":"chemical_species"},"object":{"id":"ab1a14a9-2c6b-5a60-b2ac-e65519277b78","slug":"brain-lipid-peroxidation","display_name":"Brain lipid peroxidation after carbon monoxide poisoning","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"50e97bbc-3a04-57d4-babb-9e688cbe0cd2","stable_key":"488ea171-36b8-5ef6-b5d3-98563980fdbf:hbot-pressure-alone-ineffective-event","event_type":"observed_intervention","label":"Pressure by itself did nothing; it is the oxygen at pressure that matters.","description":"Exposing rats to 3 ATA pressure with 0.21 ATA oxygen after poisoning had no significant effects, and 1 ATA oxygen did not significantly inhibit xanthine oxidase formation or lipid peroxidation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"31bd23dc-1798-52f9-b1fe-d364807d184c","slug":"chamber-pressure-alone","display_name":"Raised chamber pressure without raised oxygen fraction","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ab1a14a9-2c6b-5a60-b2ac-e65519277b78","slug":"brain-lipid-peroxidation","display_name":"Brain lipid peroxidation after carbon monoxide poisoning","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/hbot-research/8248932.abstract.txt\", \"locator\": \"Indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"54ab057eb31320490d893896d8ab5b0c856a8b59489424b14b81409f01a78f7f\", \"start_char\": 0, \"end_char\": 1870, \"text_sha256\": \"54ab057eb31320490d893896d8ab5b0c856a8b59489424b14b81409f01a78f7f\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rats poisoned with carbon monoxide, with leukocyte function assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"3 atmospheres absolute for 45 minutes, given 24 hours before or up to 45 minutes after poisoning; 3 ATA pressure without oxygen as a control","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The pressure-only control separates oxygen from pressure. The integrin defect is functional: surface expression was normal, which is the informative detail.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Hyperbaric oxygen research collection; topical membership is not evidence of a direct clinical effect, and a therapeutic exposure is not a dietary intake.","comparator":null,"unit":null,"notes":"","entity":{"slug":"hyperbaric-oxygen","display_name":"Hyperbaric oxygen therapy","entity_type_key":"drug"}},{"dimension":"organism","value_text":"Rat","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Pressure by itself did nothing; it is the oxygen at pressure that matters.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[hbot-p8248932] Functional inhibition of leukocyte B2 integrins by hyperbaric oxygen in carbon monoxide-mediated brain injury in rats. (1993). https://pubmed.ncbi.nlm.nih.gov/8248932/ DOI: 10.1006/taap.1993.1243","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Brain microvasculature and polymorphonuclear leukocytes","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"124c82cb-fe15-5b3d-82a0-af3a806d224b","evidence_kind":"source_excerpt","locator":"Lines 777-788","start_line":777,"end_line":788,"excerpt":"### hbot-pressure-alone-ineffective\nExposing rats to 3 ATA pressure with 0.21 ATA oxygen after poisoning had no significant effects, and 1 ATA oxygen did not significantly inhibit xanthine oxidase formation or lipid peroxidation.\nCondition category: normal\nnutrient_topic: Hyperbaric oxygen research collection; topical membership is not evidence of a direct clinical effect, and a therapeutic exposure is not a dietary intake.\nplain_language: Pressure by itself did nothing; it is the oxygen at pressure that matters.\norganism: Rat\ntissue_or_cell_type: Brain microvasculature and polymorphonuclear leukocytes\nexperimental_model: Rats poisoned with carbon monoxide, with leukocyte function assays\nlimitations: The pressure-only control separates oxygen from pressure. The integrin defect is functional: surface expression was normal, which is the informative detail.\nexposure: 3 atmospheres absolute for 45 minutes, given 24 hours before or up to 45 minutes after poisoning; 3 ATA pressure without oxygen as a control\nevidence_span: {\"source_cache\": \"artifacts/hbot-research/8248932.abstract.txt\", \"locator\": \"Indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"54ab057eb31320490d893896d8ab5b0c856a8b59489424b14b81409f01a78f7f\", \"start_char\": 0, \"end_char\": 1870, \"text_sha256\": \"54ab057eb31320490d893896d8ab5b0c856a8b59489424b14b81409f01a78f7f\"}\n[hbot-p8248932] Functional inhibition of leukocyte B2 integrins by hyperbaric oxygen in carbon monoxide-mediated brain injury in rats. (1993). https://pubmed.ncbi.nlm.nih.gov/8248932/ DOI: 10.1006/taap.1993.1243","model_system":"Rats poisoned with carbon monoxide, with leukocyte function assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [hbot-p8248932] Functional inhibition of leukocyte B2 integrins by hyperbaric oxygen in carbon monoxide-mediated brain injury in rats. 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