{"id":"ce51b3b5-b72d-5303-b8d7-80c08914e8ba","stable_key":"ae1ed35d-8ce7-5936-9067-a641374bbf79:carnosine-hne-adduction","predicate":"forms_adducts","statement":"Carnosine reacted with HNE to form a stabilized Michael adduct and an imine macrocycle in a pH-dependent equilibrium.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"e55b9895-3bbc-5f6e-866c-16b558ca8590","mechanism_event_label":"The peptide can chemically trap a lipid-derived aldehyde.","subject":{"id":"9998eb9b-0d09-58c9-885b-a442477dec8d","slug":"carnosine","display_name":"L-Carnosine / beta-alanyl-L-histidine","entity_type_key":"small_molecule"},"object":{"id":"fc2c1010-aab6-562d-8ba5-9a8955e1923d","slug":"carnosine-hne-adducts","display_name":"Carnosine-HNE adducts","entity_type_key":"chemical_species"},"evidence_count":1,"mechanism_event":{"id":"e55b9895-3bbc-5f6e-866c-16b558ca8590","stable_key":"ae1ed35d-8ce7-5936-9067-a641374bbf79:carnosine-hne-adduction-event","event_type":"observed_relationship","label":"The peptide can chemically trap a lipid-derived aldehyde.","description":"Carnosine reacted with HNE to form a stabilized Michael adduct and an imine macrocycle in a pH-dependent equilibrium.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9998eb9b-0d09-58c9-885b-a442477dec8d","slug":"carnosine","display_name":"L-Carnosine / beta-alanyl-L-histidine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"fc2c1010-aab6-562d-8ba5-9a8955e1923d","slug":"carnosine-hne-adducts","display_name":"Carnosine-HNE adducts","entity_type_key":"chemical_species"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"cc9bce9d-a834-5e6d-85a5-b873b8e69a21","slug":"4-hydroxynonenal","display_name":"4-Hydroxy-2-nonenal / HNE","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"16a9848a-023b-5828-9fd1-e28b9eadb901","slug":"hydrogen-ion","display_name":"Hydrogen ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Buffered chemical reactions; adducts also detected in oxidized rat muscle homogenate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Reaction chemistry does not measure clinical protection.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Carnosine collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"carnosine","display_name":"L-Carnosine / beta-alanyl-L-histidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The peptide can chemically trap a lipid-derived aldehyde.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Carnosine is a quencher of 4-hydroxy-nonenal: through what mechanism of reaction? · 2002 · https://pubmed.ncbi.nlm.nih.gov/12419310/ · DOI 10.1016/s0006-291x(02)02545-7","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"8fa1ff5d-2781-5f04-988f-ea330310ed9f","evidence_kind":"source_excerpt","locator":"Lines 236-242","start_line":236,"end_line":242,"excerpt":"## carnosine-hne-adduction\nThe peptide can chemically trap a lipid-derived aldehyde.\nCarnosine reacted with HNE to form a stabilized Michael adduct and an imine macrocycle in a pH-dependent equilibrium.\nModel: Buffered chemical reactions; adducts also detected in oxidized rat muscle homogenate.\nLimitations: Reaction chemistry does not measure clinical protection.\nEvidence access: Primary abstract\nCarnosine is a quencher of 4-hydroxy-nonenal: through what mechanism of reaction? · 2002 · https://pubmed.ncbi.nlm.nih.gov/12419310/ · DOI 10.1016/s0006-291x(02)02545-7","model_system":"Buffered chemical reactions; adducts also detected in oxidized rat muscle homogenate.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"87dc5af2-4eb6-5980-b7e0-bf3697b1156e","stable_key":"import-ae1ed35d-8ce7-5936-9067-a641374bbf79","title":"Carnosine: synthesis, transport, carbonyl chemistry and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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