{"id":"ce458f99-1c0f-5338-9626-be884a33f41b","stable_key":"31b1baa4-4113-5541-b9e7-fe44a5253a07:opc-mbp","predicate":"increases_in_recorded_experiment","statement":"SA increased MBP-associated OPC differentiation after 72 hours.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"2433046b-5aa8-5699-beea-8e778d1a8497","mechanism_event_label":"SA increased MBP-associated OPC differentiation after 72 hours.","subject":{"id":"67ad4a8f-1bf6-59e5-90a6-97b7709024d3","slug":"shikimic-acid","display_name":"Shikimic acid","entity_type_key":"small_molecule"},"object":{"id":"f6d3d9d8-1cba-59d7-973a-b7decec87431","slug":"rat-mbp","display_name":"Rat myelin basic protein / Mbp","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"2433046b-5aa8-5699-beea-8e778d1a8497","stable_key":"31b1baa4-4113-5541-b9e7-fe44a5253a07:opc-mbp-event","event_type":"experimental_observation","label":"SA increased MBP-associated OPC differentiation after 72 hours.","description":"**Oligodendrocyte differentiation differs from proliferation.** In primary rat oligodendrocyte precursor cultures, shikimic acid at 1–100 µg/mL increased MBP-associated differentiation after 72 h, with 100 µg/mL used for subsequent experiments. That highest concentration is calculated as about 574 µM. BrdU incorporation and TUNEL readouts did not significantly change. Increased MBP and fewer NG2-positive cells therefore support maturation in this model, not universal stimulation of cell proliferation. [Shikimic Acid Promotes Oligodendrocyte Precursor Cell Differentiation and Accelerates Remyelination in Mice.](https://pubmed.ncbi.nlm.nih.gov/30684125/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"67ad4a8f-1bf6-59e5-90a6-97b7709024d3","slug":"shikimic-acid","display_name":"Shikimic acid","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"Shikimic acid","sequence_order":0,"notes":""},{"entity":{"id":"f6d3d9d8-1cba-59d7-973a-b7decec87431","slug":"rat-mbp","display_name":"Rat myelin basic protein / Mbp","entity_type_key":"protein"},"role":"measured outcome","stoichiometry":null,"state_label":"increase","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text retrieved; relevant methods/results/figures reviewed. Selective extraction, not raw-data reanalysis or exhaustive supplemental extraction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Shikimic acid\", \"comparator\": \"Vehicle\", \"endpoint\": \"SA increased MBP-associated OPC differentiation after 72 hours.\", \"effect_direction\": \"increase\", \"combination\": \"single\", \"conditions\": []}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Primary rat OPCs, 1–100 µg/mL; 100 µg/mL (about 574 µM) used for follow-up.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Differentiation marker, not all-cell proliferation or human therapeutic exposure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"SA increased MBP-associated OPC differentiation after 72 hours.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Shikimic Acid Promotes Oligodendrocyte Precursor Cell Differentiation and Accelerates Remyelination in Mice. | 2019 | DOI 10.1007/s12264-018-0322-7 | PMID 30684125 | https://pubmed.ncbi.nlm.nih.gov/30684125/ | https://doi.org/10.1007/s12264-018-0322-7 | https://pmc.ncbi.nlm.nih.gov/articles/PMC6527532/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 69-69; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"640484e5-1f7e-5a91-a027-dd69e5bb431c","evidence_kind":"source_excerpt","locator":"Lines 69-69","start_line":69,"end_line":69,"excerpt":"**Oligodendrocyte differentiation differs from proliferation.** In primary rat oligodendrocyte precursor cultures, shikimic acid at 1–100 µg/mL increased MBP-associated differentiation after 72 h, with 100 µg/mL used for subsequent experiments. That highest concentration is calculated as about 574 µM. BrdU incorporation and TUNEL readouts did not significantly change. Increased MBP and fewer NG2-positive cells therefore support maturation in this model, not universal stimulation of cell proliferation. [Shikimic Acid Promotes Oligodendrocyte Precursor Cell Differentiation and Accelerates Remyelination in Mice.](https://pubmed.ncbi.nlm.nih.gov/30684125/)","model_system":"Primary rat OPCs, 1–100 µg/mL; 100 µg/mL (about 574 µM) used for follow-up.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. 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