{"id":"ce4242be-7cfd-5737-b7e2-566e6a2124c4","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:transport-slc25a32-riboflavin-selectivity","predicate":"does-not-reduce","statement":"The tested Slc25a32 mutant muscle mitochondria did not show impaired uptake of isotope-labeled free riboflavin.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"ca8ed709-278b-59c6-b8c1-09417a49bd51","mechanism_event_label":"A mitochondrial FAD transport defect need not be a free-riboflavin import defect.","subject":{"id":"c5bed2fd-37aa-54ec-ac7d-05d9f90e6d06","slug":"mouse-slc25a32-k235r-genotypes","display_name":"Slc25a32 K235R-containing mouse genotypes","entity_type_key":"gene"},"object":{"id":"d374b9fb-2f07-595f-b382-722193ff2ab8","slug":"mitochondrial-riboflavin-uptake","display_name":"Mitochondrial riboflavin uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"ca8ed709-278b-59c6-b8c1-09417a49bd51","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:transport-slc25a32-riboflavin-selectivity-event","event_type":"biochemical_relationship","label":"A mitochondrial FAD transport defect need not be a free-riboflavin import defect.","description":"The tested Slc25a32 mutant muscle mitochondria did not show impaired uptake of isotope-labeled free riboflavin.","status":"provisional","compartment":{"slug":"mitochondria","display_name":"Mitochondria"},"participants":[{"entity":{"id":"62f38e8e-01cb-5e3e-b806-8c5aa333694f","slug":"mouse-slc25a32","display_name":"Mouse Slc25a32 mitochondrial carrier","entity_type_key":"protein"},"role":"affected-carrier","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"86eb1eee-a8d1-539c-8c17-0911f69b6f1b","slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"},"role":"tested-substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c5bed2fd-37aa-54ec-ac7d-05d9f90e6d06","slug":"mouse-slc25a32-k235r-genotypes","display_name":"Slc25a32 K235R-containing mouse genotypes","entity_type_key":"gene"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"d374b9fb-2f07-595f-b382-722193ff2ab8","slug":"mitochondrial-riboflavin-uptake","display_name":"Mitochondrial riboflavin uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_location","value_text":"Figure 5C","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Stable-isotope riboflavin uptake in isolated muscle mitochondria","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"10 micromolar labeled riboflavin.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The identity of the preserved riboflavin-entry route was not established.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Riboflavin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A mitochondrial FAD transport defect need not be a free-riboflavin import defect.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[transport-slc25a32-2022] Mitochondrial FAD shortage in SLC25A32 deficiency affects folate-mediated one-carbon metabolism. (2022). https://pmc.ncbi.nlm.nih.gov/articles/PMC11072207/ DOI: 10.1007/s00018-022-04404-0","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Skeletal-muscle mitochondria","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"7961c09b-8cbd-5781-986c-bb2bf71200e8","evidence_kind":"source_excerpt","locator":"Lines 503-514","start_line":503,"end_line":514,"excerpt":"### transport-slc25a32-riboflavin-selectivity\nThe tested Slc25a32 mutant muscle mitochondria did not show impaired uptake of isotope-labeled free riboflavin.\nCondition category: machinery_impairment\nnutrient_topic: Riboflavin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A mitochondrial FAD transport defect need not be a free-riboflavin import defect.\norganism: Mus musculus\ntissue_or_cell_type: Skeletal-muscle mitochondria\nexperimental_model: Stable-isotope riboflavin uptake in isolated muscle mitochondria\nlimitations: The identity of the preserved riboflavin-entry route was not established.\nexposure: 10 micromolar labeled riboflavin.\nevidence_location: Figure 5C\n[transport-slc25a32-2022] Mitochondrial FAD shortage in SLC25A32 deficiency affects folate-mediated one-carbon metabolism. 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