{"id":"ce1da801-b465-5390-891d-e35a41134d83","stable_key":"4df6a932-097e-563a-91fe-5dceec471ffd:chloride-mpo-thiocyanate","predicate":"competes_with","statement":"Myeloperoxidase favored thiocyanate kinetically and generated hypothiocyanite despite 100 mM chloride.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"353e14ed-f7c9-5dc2-ab2c-d1f9fed42f4f","mechanism_event_label":"An abundant chloride pool does not mean it is the enzyme’s only substrate.","subject":{"id":"5d54e0a3-869f-5132-82c9-4c0ff47cd400","slug":"thiocyanate-ion","display_name":"Thiocyanate ion","entity_type_key":"ion"},"object":{"id":"6f35aa38-f875-5759-b749-97a2f6480ff3","slug":"chloride-ion","display_name":"Chloride ion","entity_type_key":"ion"},"evidence_count":1,"mechanism_event":{"id":"353e14ed-f7c9-5dc2-ab2c-d1f9fed42f4f","stable_key":"4df6a932-097e-563a-91fe-5dceec471ffd:chloride-mpo-thiocyanate-event","event_type":"biochemical_relationship","label":"An abundant chloride pool does not mean it is the enzyme’s only substrate.","description":"Myeloperoxidase favored thiocyanate kinetically and generated hypothiocyanite despite 100 mM chloride.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"53f51828-4ea1-5f2e-a044-e02577272436","slug":"mpo","display_name":"Human myeloperoxidase / MPO","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3e27fd96-b277-54f3-bb7e-35b48f9622c6","slug":"hypothiocyanite","display_name":"Hypothiocyanite / OSCN−","entity_type_key":"chemical_species"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"5d54e0a3-869f-5132-82c9-4c0ff47cd400","slug":"thiocyanate-ion","display_name":"Thiocyanate ion","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"6f35aa38-f875-5759-b749-97a2f6480ff3","slug":"chloride-ion","display_name":"Chloride ion","entity_type_key":"ion"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/chloride-research/9359420.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"83dc6a264dfb45201104568ac58c76509aaa0fabca1f32efd25861146b4b38c0\", \"start_char\": 0, \"end_char\": 1700, \"text_sha256\": \"83dc6a264dfb45201104568ac58c76509aaa0fabca1f32efd25861146b4b38c0\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified-enzyme substrate kinetics","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"100 mM chloride with varying thiocyanate","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Product chemistry under assay conditions; not a recommendation to raise chloride or thiocyanate intake.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Chloride research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"chloride","display_name":"Chloride","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Human neutrophil enzyme","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"An abundant chloride pool does not mean it is the enzyme’s only substrate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[chloride-p9359420] Thiocyanate and chloride as competing substrates for myeloperoxidase. (1997). https://pubmed.ncbi.nlm.nih.gov/9359420/ DOI: 10.1042/bj3270487","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Myeloperoxidase reaction mixture","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"d30c6d70-4817-5d86-803b-679de27e09ac","evidence_kind":"source_excerpt","locator":"Lines 627-638","start_line":627,"end_line":638,"excerpt":"### chloride-mpo-thiocyanate\nMyeloperoxidase favored thiocyanate kinetically and generated hypothiocyanite despite 100 mM chloride.\nCondition category: normal\nnutrient_topic: Chloride research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: An abundant chloride pool does not mean it is the enzyme’s only substrate.\norganism: Human neutrophil enzyme\ntissue_or_cell_type: Myeloperoxidase reaction mixture\nexperimental_model: Purified-enzyme substrate kinetics\nlimitations: Product chemistry under assay conditions; not a recommendation to raise chloride or thiocyanate intake.\nexposure: 100 mM chloride with varying thiocyanate\nevidence_span: {\"source_cache\": \"artifacts/chloride-research/9359420.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"83dc6a264dfb45201104568ac58c76509aaa0fabca1f32efd25861146b4b38c0\", \"start_char\": 0, \"end_char\": 1700, \"text_sha256\": \"83dc6a264dfb45201104568ac58c76509aaa0fabca1f32efd25861146b4b38c0\"}\n[chloride-p9359420] Thiocyanate and chloride as competing substrates for myeloperoxidase. 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