{"id":"cdf80c29-1129-5d69-9871-07d5ec429356","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:k-sur1-mutations-mgadp","predicate":"impairs-mgadp-activation-of","statement":"SUR1 K719A or K1384M substitution impaired MgADP potentiation of cloned KATP currents.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"03e1c0db-267d-58f3-ab75-bd37dbd3a947","mechanism_event_label":"Damaged nucleotide machinery can disrupt potassium-channel regulation.","subject":{"id":"433355e4-20f5-5215-9f28-53a8af12c2f0","slug":"abcc8-k719a","display_name":"SUR1 K719A variant","entity_type_key":"protein_state"},"object":{"id":"724a20c7-a425-57fd-8aa0-8f5b08d9c049","slug":"kir6-2-sur1-katp-channel","display_name":"Kir6.2-SUR1 ATP-sensitive potassium channel","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"03e1c0db-267d-58f3-ab75-bd37dbd3a947","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:k-sur1-mutations-mgadp-event","event_type":"biochemical_relationship","label":"Damaged nucleotide machinery can disrupt potassium-channel regulation.","description":"SUR1 K719A or K1384M substitution impaired MgADP potentiation of cloned KATP currents.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"99f9103e-73a2-593b-8406-9e4d3c1f623c","slug":"abcc8-k1384m","display_name":"SUR1 K1384M variant","entity_type_key":"protein_state"},"role":"independently tested variant","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"a12fd6e4-95b1-5bbf-9c5a-e4b256c0c4c2","slug":"abcc8","display_name":"Sulfonylurea receptor 1 / ABCC8","entity_type_key":"protein"},"role":"wild-type regulator","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"55da4e38-e280-584e-a524-91ae4812c592","slug":"mg-adp","display_name":"Magnesium-ADP complex","entity_type_key":"chemical_species"},"role":"activating ligand","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"bb9ad1e9-5b54-54b4-ae80-fe29ce00b2e6","slug":"kcnj11","display_name":"Kir6.2 / KCNJ11","entity_type_key":"protein"},"role":"pore subunit","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"433355e4-20f5-5215-9f28-53a8af12c2f0","slug":"abcc8-k719a","display_name":"SUR1 K719A variant","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"724a20c7-a425-57fd-8aa0-8f5b08d9c049","slug":"kir6-2-sur1-katp-channel","display_name":"Kir6.2-SUR1 ATP-sensitive potassium channel","entity_type_key":"protein_complex"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"A machinery defect interrupts magnesium-nucleotide regulation without demonstrating ion deficiency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mutant versus wild-type Xenopus patches.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Engineered substitutions are not evidence of dietary K or Mg deficiency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Potassium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Xenopus expression system","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Damaged nucleotide machinery can disrupt potassium-channel regulation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[gribble-1997-sur1] The essential role of the Walker A motifs of SUR1 in K-ATP channel activation by Mg-ADP and diazoxide (1997). https://pmc.ncbi.nlm.nih.gov/articles/PMC1169713/ DOI: 10.1093/emboj/16.6.1145","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Oocyte membrane","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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Study references: [gribble-1997-sur1] The essential role of the Walker A motifs of SUR1 in K-ATP channel activation by Mg-ADP and diazoxide (1997). https://pmc.ncbi.nlm.nih.gov/articles/PMC1169713/ DOI: 10.1093/emboj/16.6.1145","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"241226ee-6b7d-5cc7-9781-e6beb8ee1982","stable_key":"import-5102beb6-9f61-500e-af10-9ac63649e0b7","title":"Potassium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"a8f89f8d84b48c0f93048dfc45f7f57a67dd9a7249968a096a14aeb03d8b7a52","revision_id":"de4ec6f4-58a1-537b-8169-97b783898df1","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}