{"id":"cdf4da50-4cdd-55c5-b3a1-d175ae131d37","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:b2-induced-erythroid-suppression","predicate":"induced_unavailability_associated_with_erythroid_suppression","statement":"All six patients developed anemia; the study described low reticulocytes and erythroid suppression with recovery during riboflavin replacement.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"02d60863-d2bf-5ef7-be17-8a62022e3fd2","mechanism_event_label":"Severe induced shortage reduced new red-cell production; the anemia was not simply assumed to be low dietary iron.","subject":{"id":"86eb1eee-a8d1-539c-8c17-0911f69b6f1b","slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"},"object":{"id":"376fcaa7-a41d-5649-90aa-fb7deac06416","slug":"erythroid-cell-production","display_name":"Erythroid cell production","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"02d60863-d2bf-5ef7-be17-8a62022e3fd2","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:b2-induced-erythroid-suppression-event","event_type":"observed_intervention","label":"Severe induced shortage reduced new red-cell production; the anemia was not simply assumed to be low dietary iron.","description":"All six patients developed anemia; the study described low reticulocytes and erythroid suppression with recovery during riboflavin replacement.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"da6825a9-c81a-5f18-bb3d-11401d04eec8","slug":"galactoflavin","display_name":"Galactoflavin","entity_type_key":"small_molecule"},"role":"antagonist","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"89bcaf42-b4ab-5760-8c2e-44eace10cee0","slug":"iron","display_name":"Iron","entity_type_key":"nutrient_element"},"role":"supplied_nutrient","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2f3a8d0b-7b92-58d2-afc9-dd26a7cd1e0e","slug":"blood-hemoglobin-concentration","display_name":"Blood hemoglobin concentration","entity_type_key":"cellular_process"},"role":"clinical_readout","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"86eb1eee-a8d1-539c-8c17-0911f69b6f1b","slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"376fcaa7-a41d-5649-90aa-fb7deac06416","slug":"erythroid-cell-production","display_name":"Erythroid cell production","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"Iron supply alone did not prevent anemia in the combined B2 restriction/antagonist experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Historical induced-depletion study in six men with inoperable cancers; no healthy randomized control group.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Low-riboflavin semisynthetic diet plus galactoflavin antagonist, followed by riboflavin replacement; three also received oral antibiotics.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Same six-patient experiment as lesion record; other nutrients were supplied but cancer and antagonist effects limit causal generalization.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Riboflavin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Severe induced shortage reduced new red-cell production; the anemia was not simply assumed to be low dietary iron.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b2-lane1964] THE RAPID INDUCTION OF HUMAN RIBOFLAVIN DEFICIENCY WITH GALACTOFLAVIN (1964). https://pubmed.ncbi.nlm.nih.gov/14135487/ DOI: 10.1172/jci104921","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Bone marrow and circulating erythrocytes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"3757ebae-511a-500e-be54-bdcc4306e598","evidence_kind":"source_excerpt","locator":"Lines 1801-1812","start_line":1801,"end_line":1812,"excerpt":"### b2-induced-erythroid-suppression\nAll six patients developed anemia; the study described low reticulocytes and erythroid suppression with recovery during riboflavin replacement.\nCondition category: nutrient_deficiency\nnutrient_topic: Riboflavin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Severe induced shortage reduced new red-cell production; the anemia was not simply assumed to be low dietary iron.\norganism: Homo sapiens\ntissue_or_cell_type: Bone marrow and circulating erythrocytes\nexperimental_model: Historical induced-depletion study in six men with inoperable cancers; no healthy randomized control group.\nlimitations: Same six-patient experiment as lesion record; other nutrients were supplied but cancer and antagonist effects limit causal generalization.\nexposure: Low-riboflavin semisynthetic diet plus galactoflavin antagonist, followed by riboflavin replacement; three also received oral antibiotics.\ncross_nutrient: Iron supply alone did not prevent anemia in the combined B2 restriction/antagonist experiment.\n[b2-lane1964] THE RAPID INDUCTION OF HUMAN RIBOFLAVIN DEFICIENCY WITH GALACTOFLAVIN (1964). https://pubmed.ncbi.nlm.nih.gov/14135487/ DOI: 10.1172/jci104921","model_system":"Historical induced-depletion study in six men with inoperable cancers; no healthy randomized control group.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b2-lane1964] THE RAPID INDUCTION OF HUMAN RIBOFLAVIN DEFICIENCY WITH GALACTOFLAVIN (1964). https://pubmed.ncbi.nlm.nih.gov/14135487/ DOI: 10.1172/jci104921","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f7c9578-82bf-5e2d-b5c4-72a79fb4f6af","stable_key":"import-548ab9d6-3a9b-5bed-879c-17d03813b636","title":"Riboflavin: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"680cb6bc8249877f2410f551807f10d390fdd719014137d7414ba3420e29228d","revision_id":"7a61e299-908d-5372-860b-99ed190f9d7a","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}