{"id":"cde44a98-c4f4-56df-8cdf-4de2a9665307","stable_key":"b428117d-3a8c-5e74-8711-168153e19545:aspartame-dipeptide-cleavage","predicate":"undergoes","statement":"Brush-border and cytosolic preparations hydrolyzed Asp-Phe; cytosolic fractionation identified one activity peak distinct from seven previously described peptidases.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"c3c0d583-1951-5acb-9522-9b8bf2594ca3","mechanism_event_label":"The amino-acid pair can be processed after reaching a different cell compartment.","subject":{"id":"4cf892ac-f3f0-5e54-994e-509758cb3ce3","slug":"alpha-l-aspartyl-l-phenylalanine","display_name":"Alpha-L-aspartyl-L-phenylalanine","entity_type_key":"small_molecule"},"object":{"id":"f3ce39ec-8e7a-5969-bd1c-517a88fe2789","slug":"human-intestinal-asp-phe-hydrolysis","display_name":"Human intestinal Asp-Phe dipeptide hydrolysis","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"c3c0d583-1951-5acb-9522-9b8bf2594ca3","stable_key":"b428117d-3a8c-5e74-8711-168153e19545:aspartame-dipeptide-cleavage-event","event_type":"observed_relationship","label":"The amino-acid pair can be processed after reaching a different cell compartment.","description":"Brush-border and cytosolic preparations hydrolyzed Asp-Phe; 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the cytosolic enzyme was not molecularly identified.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Aspartame collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The amino-acid pair can be processed after reaching a different cell compartment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Intestinal hydrolysis of aspartylphenylalanine--the metabolic product of aspartame. · 1986 · https://pubmed.ncbi.nlm.nih.gov/3743970/ · DOI 10.1016/0016-5085(86)90697-9","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"27f3e8da-57da-5edb-961b-dc28ba835509","evidence_kind":"source_excerpt","locator":"Lines 26-32","start_line":26,"end_line":32,"excerpt":"## aspartame-dipeptide-cleavage\nThe amino-acid pair can be processed after reaching a different cell compartment.\nBrush-border and cytosolic preparations hydrolyzed Asp-Phe; cytosolic fractionation identified one activity peak distinct from seven previously described peptidases.\nModel: Human intestinal mucosa and red-cell lysate fractionation.\nLimitations: Intact dipeptide transport was proposed; the cytosolic enzyme was not molecularly identified.\nEvidence access: Primary abstract\nIntestinal hydrolysis of aspartylphenylalanine--the metabolic product of aspartame. · 1986 · https://pubmed.ncbi.nlm.nih.gov/3743970/ · DOI 10.1016/0016-5085(86)90697-9","model_system":"Human intestinal mucosa and red-cell lysate fractionation.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2bba7d36-993f-5ba7-acaf-2e4d573527ed","stable_key":"import-b428117d-3a8c-5e74-8711-168153e19545","title":"Aspartame: digestion, taste, metabolite dependencies and experimental signaling (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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