{"id":"cdc00758-e712-593c-a5cd-25c57860d6bb","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-trans-renal-sodium-gradient","predicate":"drives","statement":"An inward sodium gradient drove transient pantothenate accumulation against its concentration gradient in voltage-clamped rabbit renal brush-border vesicles.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"62edbb4b-ec57-540a-9267-ab73f8581c51","mechanism_event_label":"Sodium provides energy for pantothenate uptake at the rabbit kidney brush border.","subject":{"id":"38de8704-84db-5770-ac1d-242cd787e798","slug":"sodium-ion","display_name":"Sodium ion","entity_type_key":"ion"},"object":{"id":"3d57d1cd-3617-5b54-919b-0f82f1f27a8e","slug":"rabbit-renal-brush-border-pantothenate-uptake","display_name":"Rabbit renal brush-border pantothenate uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"62edbb4b-ec57-540a-9267-ab73f8581c51","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-trans-renal-sodium-gradient-event","event_type":"biochemical_relationship","label":"Sodium provides energy for pantothenate uptake at the rabbit kidney brush border.","description":"An inward sodium gradient drove transient pantothenate accumulation against its concentration gradient in voltage-clamped rabbit renal brush-border vesicles.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"7bc62072-9517-5563-a3b8-8c5ab64a464b","slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"},"role":"transported_substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"38de8704-84db-5770-ac1d-242cd787e798","slug":"sodium-ion","display_name":"Sodium ion","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"3d57d1cd-3617-5b54-919b-0f82f1f27a8e","slug":"rabbit-renal-brush-border-pantothenate-uptake","display_name":"Rabbit renal brush-border pantothenate uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Isolated rabbit renal brush-border membrane vesicles with controlled ion gradients and membrane potentials","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Inward NaCl gradient under voltage clamp; potassium, rubidium, lithium, ammonium and choline could not substitute for sodium.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Rabbit isolated-membrane experiment; this predates molecular identification of SMVT and does not independently assign the effect to human SLC5A6 or establish a human renal clearance threshold.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Oryctolagus cuniculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Sodium provides energy for pantothenate uptake at the rabbit kidney brush border.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b5-trans-renal1986] Pantothenate-sodium cotransport in renal brush-border membranes. (1986). https://pubmed.ncbi.nlm.nih.gov/3771539/ DOI: 10.1016/s0021-9258(18)66891-7","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Renal brush-border membrane vesicles","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ae0c5377-eca6-5071-bd69-362d6f9e19d1","evidence_kind":"source_excerpt","locator":"Lines 236-247","start_line":236,"end_line":247,"excerpt":"### b5-trans-renal-sodium-gradient\nAn inward sodium gradient drove transient pantothenate accumulation against its concentration gradient in voltage-clamped rabbit renal brush-border vesicles.\nCondition category: normal\nnutrient_topic: Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Sodium provides energy for pantothenate uptake at the rabbit kidney brush border.\norganism: Oryctolagus cuniculus\ntissue_or_cell_type: Renal brush-border membrane vesicles\nexperimental_model: Isolated rabbit renal brush-border membrane vesicles with controlled ion gradients and membrane potentials\nlimitations: Rabbit isolated-membrane experiment; this predates molecular identification of SMVT and does not independently assign the effect to human SLC5A6 or establish a human renal clearance threshold.\nexposure: Inward NaCl gradient under voltage clamp; potassium, rubidium, lithium, ammonium and choline could not substitute for sodium.\ncross_nutrient: true\n[b5-trans-renal1986] Pantothenate-sodium cotransport in renal brush-border membranes. 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