{"id":"cd6a90c3-1a47-58d1-8d52-713e8736ed56","stable_key":"56f1d914-e7da-595a-af69-c217b2b47407:l-phenylalanine-microbial-oxidation","predicate":"supports_formation_of","statement":"Clostridium sporogenes porA disruption reduced the oxidative conversion of phenylalanine-derived carbon to phenylacetate; labeled phenylalanine tracing supported the pathway through phenylpyruvate.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"e17efac7-0f49-5745-b733-dfb7ca5176e5","mechanism_event_label":"Some gut bacteria send phenylalanine into a different metabolic route.","subject":{"id":"60aa0e9b-a5ee-52ce-b43e-3cadf3e1763e","slug":"c-sporogenes-pora","display_name":"Clostridium sporogenes PorA","entity_type_key":"protein"},"object":{"id":"15dd7892-f298-50d1-aaef-7f7b9f94a033","slug":"phenylacetic-acid","display_name":"Phenylacetic acid / phenylacetate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"e17efac7-0f49-5745-b733-dfb7ca5176e5","stable_key":"56f1d914-e7da-595a-af69-c217b2b47407:l-phenylalanine-microbial-oxidation-event","event_type":"observed_relationship","label":"Some gut bacteria send phenylalanine into a different metabolic route.","description":"Clostridium sporogenes porA disruption reduced the oxidative conversion of phenylalanine-derived carbon to phenylacetate; labeled phenylalanine tracing supported the pathway through phenylpyruvate.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"60aa0e9b-a5ee-52ce-b43e-3cadf3e1763e","slug":"c-sporogenes-pora","display_name":"Clostridium sporogenes PorA","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"15dd7892-f298-50d1-aaef-7f7b9f94a033","slug":"phenylacetic-acid","display_name":"Phenylacetic acid / phenylacetate","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"190407ad-0219-54b5-b05f-7c75f3895ca6","slug":"l-phenylalanine","display_name":"L-Phenylalanine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"b4319bbf-7e42-5496-af9a-8177927507a4","slug":"phenylpyruvate","display_name":"Phenylpyruvate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Defined bacterial cultures and stable-isotope experiments; 100 micromolar labeled phenylalanine over 24 hours.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Bacterial flux is strain- and environment-dependent; no human dietary conversion fraction is established.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Phenylalanine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-phenylalanine","display_name":"L-Phenylalanine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Some gut bacteria send phenylalanine into a different metabolic route.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"A Cardiovascular Disease-Linked Gut Microbial Metabolite Acts via Adrenergic Receptors. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32142679/ · DOI 10.1016/j.cell.2020.02.016","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ceada863-6635-5be2-a34e-245bba946af2","evidence_kind":"source_excerpt","locator":"Lines 342-348","start_line":342,"end_line":348,"excerpt":"## l-phenylalanine-microbial-oxidation\nSome gut bacteria send phenylalanine into a different metabolic route.\nClostridium sporogenes porA disruption reduced the oxidative conversion of phenylalanine-derived carbon to phenylacetate; labeled phenylalanine tracing supported the pathway through phenylpyruvate.\nModel: Defined bacterial cultures and stable-isotope experiments; 100 micromolar labeled phenylalanine over 24 hours.\nLimitations: Bacterial flux is strain- and environment-dependent; no human dietary conversion fraction is established.\nEvidence access: Primary full text\nA Cardiovascular Disease-Linked Gut Microbial Metabolite Acts via Adrenergic Receptors. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32142679/ · DOI 10.1016/j.cell.2020.02.016","model_system":"Defined bacterial cultures and stable-isotope experiments; 100 micromolar labeled phenylalanine over 24 hours.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"54de7c56-1e60-5d7b-8ef9-852c58ae92ba","stable_key":"import-56f1d914-e7da-595a-af69-c217b2b47407","title":"L-Phenylalanine: transport, protein synthesis, cofactor recycling and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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