{"id":"cd6a81e2-a76a-553d-93db-b177cf328291","stable_key":"31b1baa4-4113-5541-b9e7-fe44a5253a07:anisatin-current","predicate":"decreases_in_recorded_experiment","statement":"Anisatin inhibited GABA-activated current in a use-dependent manner, estimated EC50 1.10 µM.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"c6bba86e-0dc2-5a88-a889-5718a508a87f","mechanism_event_label":"Anisatin inhibited GABA-activated current in a use-dependent manner, estimated EC50 1.10 µM.","subject":{"id":"701200c1-3270-5363-a2d3-d659630d876c","slug":"anisatin","display_name":"Anisatin","entity_type_key":"small_molecule"},"object":{"id":"af4ce987-2dcd-5073-8e7d-dc67fef08d29","slug":"rat-drg-gaba-current-anisatin","display_name":"Rat DRG GABA-activated current in anisatin assays","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"c6bba86e-0dc2-5a88-a889-5718a508a87f","stable_key":"31b1baa4-4113-5541-b9e7-fe44a5253a07:anisatin-current-event","event_type":"experimental_observation","label":"Anisatin inhibited GABA-activated current in a use-dependent manner, estimated EC50 1.10 µM.","description":"**Star-anise toxicity is a preparation-identity issue.** Anisatin inhibited GABA-activated currents in rat dorsal-root-ganglion neurons in a use-dependent fashion; an estimated EC50 was 1.10 µM. It is a different molecule from shikimic acid. Seven infant neurological cases associated with star-anise tea and evidence of toxic species contamination demonstrate preparation risk, not toxicity attributable specifically to purified shikimic acid. Botanical identity, plant part, contamination, extraction and measured composition cannot be inferred from the name star anise. [Anisatin modulation of the gamma-aminobutyric acid receptor-channel in rat dorsal root ganglion neurons.](https://pubmed.ncbi.nlm.nih.gov/10455311/) [Neurotoxicities in infants seen with the consumption of star anise tea.](https://pubmed.ncbi.nlm.nih.gov/15492355/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"701200c1-3270-5363-a2d3-d659630d876c","slug":"anisatin","display_name":"Anisatin","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"Anisatin during GABA activation","sequence_order":0,"notes":""},{"entity":{"id":"af4ce987-2dcd-5073-8e7d-dc67fef08d29","slug":"rat-drg-gaba-current-anisatin","display_name":"Rat DRG GABA-activated current in anisatin assays","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"decrease","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary indexed abstract reviewed; full results, tables and supplements not independently extracted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Anisatin during GABA activation\", \"comparator\": \"GABA activation without anisatin\", \"endpoint\": \"Anisatin inhibited GABA-activated current in a use-dependent manner, estimated EC50 1.10 µM.\", \"effect_direction\": \"decrease\", \"combination\": \"single\", \"conditions\": []}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Rat dorsal-root-ganglion electrophysiology.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Different molecule from shikimic acid; not proof of parent-compound toxicity.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Anisatin inhibited GABA-activated current in a use-dependent manner, estimated EC50 1.10 µM.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Anisatin modulation of the gamma-aminobutyric acid receptor-channel in rat dorsal root ganglion neurons. | 1999 | DOI 10.1038/sj.bjp.0702700 | PMID 10455311 | https://pubmed.ncbi.nlm.nih.gov/10455311/ | https://doi.org/10.1038/sj.bjp.0702700 | https://pmc.ncbi.nlm.nih.gov/articles/PMC1566146/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Neurotoxicities in infants seen with the consumption of star anise tea. | 2004 | DOI 10.1542/peds.2004-0058 | PMID 15492355 | https://pubmed.ncbi.nlm.nih.gov/15492355/ | https://doi.org/10.1542/peds.2004-0058","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 89-89; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"d3f25c4b-b27e-5358-932e-98fcd122703e","evidence_kind":"source_excerpt","locator":"Lines 89-89","start_line":89,"end_line":89,"excerpt":"**Star-anise toxicity is a preparation-identity issue.** Anisatin inhibited GABA-activated currents in rat dorsal-root-ganglion neurons in a use-dependent fashion; an estimated EC50 was 1.10 µM. It is a different molecule from shikimic acid. Seven infant neurological cases associated with star-anise tea and evidence of toxic species contamination demonstrate preparation risk, not toxicity attributable specifically to purified shikimic acid. Botanical identity, plant part, contamination, extraction and measured composition cannot be inferred from the name star anise. [Anisatin modulation of the gamma-aminobutyric acid receptor-channel in rat dorsal root ganglion neurons.](https://pubmed.ncbi.nlm.nih.gov/10455311/) [Neurotoxicities in infants seen with the consumption of star anise tea.](https://pubmed.ncbi.nlm.nih.gov/15492355/)","model_system":"Rat dorsal-root-ganglion electrophysiology.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. Not a verbatim quotation from a primary paper.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e6ae59de-0369-5c2f-8262-57d91302671c","stable_key":"import-31b1baa4-4113-5541-b9e7-fe44a5253a07","title":"Shikimic acid: detailed mechanisms of action (reviewed 5 October 2026)","document_type":"imported_text","citation_label":"Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication.","file_path":"","sha256":"95b1f9e9577661312d67f36e156d2e49326b207f296c1c0e801a5b007fd8e283","revision_id":"cd3237f1-131a-557d-84b5-7543259807b0","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}