{"id":"cd1621b5-169c-5114-ba0d-a8bed2a2753a","stable_key":"cfa4bd86-d88d-555a-975c-68877bf00e0f:lutein-srbi-cell-uptake","predicate":"increases","statement":"SR-BI expression increased lutein and zeaxanthin uptake preferentially over beta-carotene.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"4d234d87-e257-5a5b-ac08-4ac876c36c3e","mechanism_event_label":"The receptor changed which pigments entered these cells.","subject":{"id":"e00491c3-4448-559c-9012-57f5ce969157","slug":"scarb1","display_name":"Scavenger receptor class B type 1 / SCARB1","entity_type_key":"protein"},"object":{"id":"0991b9e6-6d8a-5cf2-8b8f-b04948da294c","slug":"hek293-lutein-uptake","display_name":"Lutein uptake by engineered human HEK293 cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"4d234d87-e257-5a5b-ac08-4ac876c36c3e","stable_key":"cfa4bd86-d88d-555a-975c-68877bf00e0f:lutein-srbi-cell-uptake-event","event_type":"biochemical_relationship","label":"The receptor changed which pigments entered these cells.","description":"SR-BI expression increased lutein and zeaxanthin uptake preferentially over beta-carotene.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"5dd84367-3f8e-5a3f-b952-a96174ea51fa","slug":"lutein","display_name":"Lutein","entity_type_key":"small_molecule"},"role":"cargo","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e00491c3-4448-559c-9012-57f5ce969157","slug":"scarb1","display_name":"Scavenger receptor class B type 1 / SCARB1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"0991b9e6-6d8a-5cf2-8b8f-b04948da294c","slug":"hek293-lutein-uptake","display_name":"Lutein uptake by engineered human HEK293 cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/lutein-research/36863431.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"530dd42d442890be2e5cd11c18bf95c0bd850d8f67ad6682d12360dfc7f8aec0\", \"start_char\": 0, \"end_char\": 1551, \"text_sha256\": \"530dd42d442890be2e5cd11c18bf95c0bd850d8f67ad6682d12360dfc7f8aec0\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"SR-BI expression, tunnel mutation, binding and lipoprotein-partner assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Wild-type or C384Y SR-BI; HDL and hepatic lipase additions","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Engineered-cell mechanisms, not a clinical HDL-raising recommendation; no selective direct binding to lutein demonstrated.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Lutein research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"lutein","display_name":"Lutein","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human HEK293 cell system","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The receptor changed which pigments entered these cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[lutein-p36863431] Mechanism for the selective uptake of macular carotenoids mediated by the HDL cholesterol receptor SR-BI. (2023). https://pubmed.ncbi.nlm.nih.gov/36863431/ DOI: 10.1016/j.exer.2023.109429","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Engineered cells without endogenous SR-BI","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"1466fb5c-bbef-5e16-a641-ce9b78ba4d25","evidence_kind":"source_excerpt","locator":"Lines 671-682","start_line":671,"end_line":682,"excerpt":"### lutein-srbi-cell-uptake\nSR-BI expression increased lutein and zeaxanthin uptake preferentially over beta-carotene.\nCondition category: normal\nnutrient_topic: Lutein research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The receptor changed which pigments entered these cells.\norganism: Human HEK293 cell system\ntissue_or_cell_type: Engineered cells without endogenous SR-BI\nexperimental_model: SR-BI expression, tunnel mutation, binding and lipoprotein-partner assays\nlimitations: Engineered-cell mechanisms, not a clinical HDL-raising recommendation; no selective direct binding to lutein demonstrated.\nexposure: Wild-type or C384Y SR-BI; HDL and hepatic lipase additions\nevidence_span: {\"source_cache\": \"artifacts/lutein-research/36863431.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"530dd42d442890be2e5cd11c18bf95c0bd850d8f67ad6682d12360dfc7f8aec0\", \"start_char\": 0, \"end_char\": 1551, \"text_sha256\": \"530dd42d442890be2e5cd11c18bf95c0bd850d8f67ad6682d12360dfc7f8aec0\"}\n[lutein-p36863431] Mechanism for the selective uptake of macular carotenoids mediated by the HDL cholesterol receptor SR-BI. 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