{"id":"cd11a8d6-33a3-58fb-b5f6-2e9a5d81a30e","stable_key":"4d8e4781-3878-5818-8d0a-7dbd6e024555:chromium-membrane-cholesterol-protection","predicate":"limits","statement":"Chromium picolinate protected hyperinsulinemic L6 myotubes against excess membrane cholesterol.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"7ce058fb-44ff-5005-a8ce-041e044b90a2","mechanism_event_label":"The treatment prevented a membrane-lipid change associated with insulin resistance in this model.","subject":{"id":"89d5d39c-2663-5e0c-8eae-562a5b25a47d","slug":"chromium-iii-picolinate","display_name":"Chromium(III) picolinate / CrPic","entity_type_key":"chemical_species"},"object":{"id":"5dd96b45-fcf8-5cb5-8392-b3f64c2ce74b","slug":"plasma-membrane-cholesterol-content","display_name":"Plasma-membrane cholesterol content","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"7ce058fb-44ff-5005-a8ce-041e044b90a2","stable_key":"4d8e4781-3878-5818-8d0a-7dbd6e024555:chromium-membrane-cholesterol-protection-event","event_type":"biochemical_relationship","label":"The treatment prevented a membrane-lipid change associated with insulin resistance in this model.","description":"Chromium picolinate protected hyperinsulinemic L6 myotubes against excess membrane cholesterol.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"d3a1b93d-0a2c-5d1b-b672-5e4ef4b09a10","slug":"cholesterol","display_name":"Cholesterol","entity_type_key":"lipid"},"role":"measured_lipid","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"89d5d39c-2663-5e0c-8eae-562a5b25a47d","slug":"chromium-iii-picolinate","display_name":"Chromium(III) picolinate / CrPic","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"5dd96b45-fcf8-5cb5-8392-b3f64c2ce74b","slug":"plasma-membrane-cholesterol-content","display_name":"Plasma-membrane cholesterol content","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/chromium-research/24725432.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"f172a147ded27e509e27d5bcc53ad6dc3e8b22b424ab021515b205ae29bccbc4\", \"start_char\": 0, \"end_char\": 1788, \"text_sha256\": \"f172a147ded27e509e27d5bcc53ad6dc3e8b22b424ab021515b205ae29bccbc4\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Hyperinsulinemia model with AMPK silencing","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Chromium picolinate during chronic low-dose insulin exposure, followed by acute insulin response; AMPK siRNA","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell-culture mechanism. The indexed abstract identifies AMPK depletion without resolving the targeted catalytic isoform; family-level identity is retained. This is not a dietary repletion or human treatment experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Chromium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"chromium","display_name":"Chromium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Rat L6 skeletal muscle myotubes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The treatment prevented a membrane-lipid change associated with insulin resistance in this model.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[chromium-p24725432] Chromium enhances insulin responsiveness via AMPK. 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The indexed abstract identifies AMPK depletion without resolving the targeted catalytic isoform; family-level identity is retained. This is not a dietary repletion or human treatment experiment.\nexposure: Chromium picolinate during chronic low-dose insulin exposure, followed by acute insulin response; AMPK siRNA\nevidence_span: {\"source_cache\": \"artifacts/chromium-research/24725432.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"f172a147ded27e509e27d5bcc53ad6dc3e8b22b424ab021515b205ae29bccbc4\", \"start_char\": 0, \"end_char\": 1788, \"text_sha256\": \"f172a147ded27e509e27d5bcc53ad6dc3e8b22b424ab021515b205ae29bccbc4\"}\n[chromium-p24725432] Chromium enhances insulin responsiveness via AMPK. 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