{"id":"ccb3018a-7f47-5a3e-b319-b557ec493594","stable_key":"22527305-af1f-536a-b419-7e73f08cc3ec:luteolin-platelet-stimulus","predicate":"has_stimulus_dependent_effect","statement":"At 25 micromolar, luteolin did not significantly inhibit ADP- or U46619-induced aggregation in the short-incubation protocol.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"9242e060-26a2-5003-85da-a475bff4df1f","mechanism_event_label":"A negative result limits the idea of universal platelet blockade.","subject":{"id":"80472c4b-ecc2-52e3-a766-f8463294774f","slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"},"object":{"id":"edd55530-17a3-54ad-8678-a0617cc1d72b","slug":"human-platelet-agonist-response","display_name":"Human platelet agonist-specific aggregation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"9242e060-26a2-5003-85da-a475bff4df1f","stable_key":"22527305-af1f-536a-b419-7e73f08cc3ec:luteolin-platelet-stimulus-event","event_type":"observed_relationship","label":"A negative result limits the idea of universal platelet blockade.","description":"At 25 micromolar, luteolin did not significantly inhibit ADP- or U46619-induced aggregation in the short-incubation protocol.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"80472c4b-ecc2-52e3-a766-f8463294774f","slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"edd55530-17a3-54ad-8678-a0617cc1d72b","slug":"human-platelet-agonist-response","display_name":"Human platelet agonist-specific aggregation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Washed human platelets; 10-minute pretreatment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Higher concentrations and longer incubation can differ.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Luteolin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A negative result limits the idea of universal platelet blockade.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Luteolin inhibits GPVI-mediated platelet activation, oxidative stress, and thrombosis. · 2023 · https://pubmed.ncbi.nlm.nih.gov/38026984/ · DOI 10.3389/fphar.2023.1255069","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"eabdd23c-f2bb-5da2-9935-496973ac8a56","evidence_kind":"source_excerpt","locator":"Lines 444-450","start_line":444,"end_line":450,"excerpt":"## luteolin-platelet-stimulus\nA negative result limits the idea of universal platelet blockade.\nAt 25 micromolar, luteolin did not significantly inhibit ADP- or U46619-induced aggregation in the short-incubation protocol.\nModel: Washed human platelets; 10-minute pretreatment.\nLimitations: Higher concentrations and longer incubation can differ.\nEvidence access: Primary full text\nLuteolin inhibits GPVI-mediated platelet activation, oxidative stress, and thrombosis. · 2023 · https://pubmed.ncbi.nlm.nih.gov/38026984/ · DOI 10.3389/fphar.2023.1255069","model_system":"Washed human platelets; 10-minute pretreatment.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"26337a08-5cee-5cee-900e-e6d3e26ac6e4","stable_key":"import-22527305-af1f-536a-b419-7e73f08cc3ec","title":"Luteolin: metabolism, immune signaling, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"fd6ea0b1cef8d1c9965270494bfa815de14f679044f0b5d5d7bbf4904630ad76","revision_id":"c9a3dd80-c7e6-5fb4-bc91-8db265e7c8de","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}