{"id":"cc7f8348-881a-5374-b4da-8296021fccb9","stable_key":"0ad8610d-d575-5870-b7cd-763a9f750783:copper-atox1-atp7a-traffic","predicate":"loss_impairs","statement":"Atox1 loss impaired copper-responsive ATP7A movement from the Golgi even when intracellular copper concentrations were matched.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"abab6d95-0de6-5402-be46-058b8aef517d","mechanism_event_label":"The copper courier affects transporter behavior as well as total copper content.","subject":{"id":"a8a3abda-8a13-53e6-8a64-b27d930e36cc","slug":"mouse-atox1","display_name":"Mouse copper chaperone Atox1","entity_type_key":"protein"},"object":{"id":"64f3611c-b24f-5b1a-803b-4e6538c3e5f1","slug":"atp7a-golgi-exit","display_name":"Copper-responsive ATP7A exit from the Golgi","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"abab6d95-0de6-5402-be46-058b8aef517d","stable_key":"0ad8610d-d575-5870-b7cd-763a9f750783:copper-atox1-atp7a-traffic-event","event_type":"biochemical_relationship","label":"The copper courier affects transporter behavior as well as total copper content.","description":"Atox1 loss impaired copper-responsive ATP7A movement from the Golgi even when intracellular copper concentrations were matched.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4402b6dd-887c-52c1-befa-789a47b8dc38","slug":"mouse-atp7a","display_name":"Mouse copper-transporting ATPase Atp7a","entity_type_key":"protein"},"role":"affected transporter","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"a8a3abda-8a13-53e6-8a64-b27d930e36cc","slug":"mouse-atox1","display_name":"Mouse copper chaperone Atox1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"64f3611c-b24f-5b1a-803b-4e6538c3e5f1","slug":"atp7a-golgi-exit","display_name":"Copper-responsive ATP7A exit from the Golgi","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/copper-research/12538877.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"480f54240ace7abfb1e0a00ae4f113de3bb518ae356fc523354ced9619428283\", \"start_char\": 0, \"end_char\": 1579, \"text_sha256\": \"480f54240ace7abfb1e0a00ae4f113de3bb518ae356fc523354ced9619428283\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Atox1-null and control immortalized embryonic fibroblasts","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Atox1 deletion and copper challenge","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Trafficking and efflux are related but distinguishable; this is a cell knockout study, not human dietary depletion.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Copper research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"copper","display_name":"Copper","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mouse","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The copper courier affects transporter behavior as well as total copper content.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[copper-p12538877] Essential role for Atox1 in the copper-mediated intracellular trafficking of the Menkes ATPase. (2003). https://pubmed.ncbi.nlm.nih.gov/12538877/ DOI: 10.1073/pnas.0336230100","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Fibroblast Golgi and cellular copper pools","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"868cbe1c-d175-56ae-9fbc-b75560771138","evidence_kind":"source_excerpt","locator":"Lines 377-388","start_line":377,"end_line":388,"excerpt":"### copper-atox1-atp7a-traffic\nAtox1 loss impaired copper-responsive ATP7A movement from the Golgi even when intracellular copper concentrations were matched.\nCondition category: machinery_impairment\nnutrient_topic: Copper research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The copper courier affects transporter behavior as well as total copper content.\norganism: Mouse\ntissue_or_cell_type: Fibroblast Golgi and cellular copper pools\nexperimental_model: Atox1-null and control immortalized embryonic fibroblasts\nlimitations: Trafficking and efflux are related but distinguishable; this is a cell knockout study, not human dietary depletion.\nexposure: Atox1 deletion and copper challenge\nevidence_span: {\"source_cache\": \"artifacts/copper-research/12538877.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"480f54240ace7abfb1e0a00ae4f113de3bb518ae356fc523354ced9619428283\", \"start_char\": 0, \"end_char\": 1579, \"text_sha256\": \"480f54240ace7abfb1e0a00ae4f113de3bb518ae356fc523354ced9619428283\"}\n[copper-p12538877] Essential role for Atox1 in the copper-mediated intracellular trafficking of the Menkes ATPase. 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