{"id":"cc722607-83e0-534e-81d8-3baafbcfeaeb","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-met-acc-acetylcoa-binding","predicate":"binds_to","statement":"The inactive human ACC1 filament structure resolved acetyl-CoA in a pocket at the carboxyltransferase dimer interface.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"df37687d-98d9-56db-af97-c187bf318df2","mechanism_event_label":"Acetyl-CoA binds the biotin-dependent carboxylase even in a structurally inactive state.","subject":{"id":"b7ed6e4c-e560-5cee-b68f-895f21862e6f","slug":"acetyl-coa","display_name":"Acetyl-CoA","entity_type_key":"small_molecule"},"object":{"id":"ae7b709a-c5e2-5814-af16-920bddacc7c7","slug":"acaca","display_name":"Human acetyl-CoA carboxylase 1 / ACACA","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"df37687d-98d9-56db-af97-c187bf318df2","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-met-acc-acetylcoa-binding-event","event_type":"biochemical_relationship","label":"Acetyl-CoA binds the biotin-dependent carboxylase even in a structurally inactive state.","description":"The inactive human ACC1 filament structure resolved acetyl-CoA in a pocket at the carboxyltransferase dimer interface.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"37a8e96b-f95b-5ba7-a0bc-8ed3cfaf5fd8","slug":"biotin","display_name":"Biotin","entity_type_key":"small_molecule"},"role":"covalently_bound_enzyme_cofactor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2ba24c3a-760f-5ff8-ac6b-1e74d03cd070","slug":"acaca-biotinyl-k786","display_name":"Human ACC1 biotinylated at Lys786","entity_type_key":"protein_state"},"role":"normal_biotinylated_protein_state","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b7ed6e4c-e560-5cee-b68f-895f21862e6f","slug":"acetyl-coa","display_name":"Acetyl-CoA","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"ae7b709a-c5e2-5814-af16-920bddacc7c7","slug":"acaca","display_name":"Human acetyl-CoA carboxylase 1 / ACACA","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Full text lines 101, 131; Fig. 4B","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Endogenous human ACC1 purified from Expi293F cells and analyzed by cryo-EM","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Substrate preparation contained 10 mM acetyl-CoA, 25 mM bicarbonate, 10 mM MgCl2 and 10 mM ATP; cryo-EM preparation conditions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Ligand occupancy in an inactive structure is not an activity or dietary-repletion assay. Biotin density was not resolved in this map. This experiment does not establish a dietary pantothenate threshold or benefit from B5 supplementation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Acetyl-CoA binds the biotin-dependent carboxylase even in a structurally inactive state.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b5-met-acc2024] Filament structures unveil the dynamic organization of human acetyl-CoA carboxylase. (2024). https://pubmed.ncbi.nlm.nih.gov/39383219/ DOI: 10.1126/sciadv.ado4880","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified endogenous Expi293F ACC1","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"488b3cce-c60c-5cb1-be31-82d9e2aa84bd","evidence_kind":"source_excerpt","locator":"Lines 1033-1045","start_line":1033,"end_line":1045,"excerpt":"### b5-met-acc-acetylcoa-binding\nThe inactive human ACC1 filament structure resolved acetyl-CoA in a pocket at the carboxyltransferase dimer interface.\nCondition category: normal\nnutrient_topic: Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Acetyl-CoA binds the biotin-dependent carboxylase even in a structurally inactive state.\norganism: Homo sapiens\ntissue_or_cell_type: Purified endogenous Expi293F ACC1\nexperimental_model: Endogenous human ACC1 purified from Expi293F cells and analyzed by cryo-EM\nlimitations: Ligand occupancy in an inactive structure is not an activity or dietary-repletion assay. Biotin density was not resolved in this map. This experiment does not establish a dietary pantothenate threshold or benefit from B5 supplementation.\nexposure: Substrate preparation contained 10 mM acetyl-CoA, 25 mM bicarbonate, 10 mM MgCl2 and 10 mM ATP; cryo-EM preparation conditions.\ncross_nutrient: true\nevidence_location: Full text lines 101, 131; Fig. 4B\n[b5-met-acc2024] Filament structures unveil the dynamic organization of human acetyl-CoA carboxylase. (2024). https://pubmed.ncbi.nlm.nih.gov/39383219/ DOI: 10.1126/sciadv.ado4880","model_system":"Endogenous human ACC1 purified from Expi293F cells and analyzed by cryo-EM","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b5-met-acc2024] Filament structures unveil the dynamic organization of human acetyl-CoA carboxylase. (2024). https://pubmed.ncbi.nlm.nih.gov/39383219/ DOI: 10.1126/sciadv.ado4880","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"0716b500-dd0b-5425-b9d0-d88bc9c09e86","stable_key":"import-f992b796-377d-53bf-a39b-7e5d41dbe194","title":"Pantothenic acid (vitamin B5): coenzyme A, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"b5a49f5a2373ca6064c9a4e014e052dad2f6f199a82bb09b26b82b831c36110b","revision_id":"78f3e793-f084-5ee2-8703-b661c4b650bf","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}