{"id":"cc5919b1-fa77-5c0c-ad82-ce2bf94ce249","stable_key":"27e0c1cf-7726-5164-8b15-27a631584cd0:choline-aldh9-betaine","predicate":"oxidizes","statement":"Purified human E3 aldehyde dehydrogenase showed betaine-aldehyde dehydrogenase activity, with the activities co-purifying across six columns.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"f665593d-e55e-571e-961e-7a897192a479","mechanism_event_label":"This enzyme can process the aldehyde intermediate in the betaine pathway.","subject":{"id":"c31af957-2ab3-5b80-b389-8989755433e4","slug":"aldh9a1","display_name":"ALDH9A1","entity_type_key":"protein"},"object":{"id":"2922542d-d263-5f46-afde-503b30e4940d","slug":"betaine-aldehyde","display_name":"Betaine aldehyde","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"f665593d-e55e-571e-961e-7a897192a479","stable_key":"27e0c1cf-7726-5164-8b15-27a631584cd0:choline-aldh9-betaine-event","event_type":"biochemical_relationship","label":"This enzyme can process the aldehyde intermediate in the betaine pathway.","description":"Purified human E3 aldehyde dehydrogenase showed betaine-aldehyde dehydrogenase activity, with the activities co-purifying across six columns.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f2ed20a5-c46b-5d60-9368-35d4969b6980","slug":"betaine","display_name":"Betaine","entity_type_key":"small_molecule"},"role":"reaction_product","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"c31af957-2ab3-5b80-b389-8989755433e4","slug":"aldh9a1","display_name":"ALDH9A1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2922542d-d263-5f46-afde-503b30e4940d","slug":"betaine-aldehyde","display_name":"Betaine aldehyde","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/choline-research/7646513.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"26a654f5b886888ad8c9de61fc59a9fcbaa08dce54a8bd2ba4be43ac637071dc\", \"start_char\": 0, \"end_char\": 687, \"text_sha256\": \"26a654f5b886888ad8c9de61fc59a9fcbaa08dce54a8bd2ba4be43ac637071dc\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified human E3 aldehyde dehydrogenase substrate assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Betaine aldehyde and 4-aminobutyraldehyde activity across six chromatography steps","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"E3/ALDH9A1 has demonstrated betaine-aldehyde activity; not an exclusive assignment of every tissue’s betaine synthesis to this enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Choline research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"choline","display_name":"Choline","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human enzyme","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"This enzyme can process the aldehyde intermediate in the betaine pathway.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[choline-p7646513] Human aldehyde dehydrogenase E3 isozyme is a betaine aldehyde dehydrogenase. (1995). https://pubmed.ncbi.nlm.nih.gov/7646513/ DOI: 10.1006/bbrc.1995.2168","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified protein","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"3af7d15f-20b7-52f1-966a-0f203549c408","evidence_kind":"source_excerpt","locator":"Lines 607-618","start_line":607,"end_line":618,"excerpt":"### choline-aldh9-betaine\nPurified human E3 aldehyde dehydrogenase showed betaine-aldehyde dehydrogenase activity, with the activities co-purifying across six columns.\nCondition category: normal\nnutrient_topic: Choline research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: This enzyme can process the aldehyde intermediate in the betaine pathway.\norganism: Human enzyme\ntissue_or_cell_type: Purified protein\nexperimental_model: Purified human E3 aldehyde dehydrogenase substrate assays\nlimitations: E3/ALDH9A1 has demonstrated betaine-aldehyde activity; not an exclusive assignment of every tissue’s betaine synthesis to this enzyme.\nexposure: Betaine aldehyde and 4-aminobutyraldehyde activity across six chromatography steps\nevidence_span: {\"source_cache\": \"artifacts/choline-research/7646513.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"26a654f5b886888ad8c9de61fc59a9fcbaa08dce54a8bd2ba4be43ac637071dc\", \"start_char\": 0, \"end_char\": 687, \"text_sha256\": \"26a654f5b886888ad8c9de61fc59a9fcbaa08dce54a8bd2ba4be43ac637071dc\"}\n[choline-p7646513] Human aldehyde dehydrogenase E3 isozyme is a betaine aldehyde dehydrogenase. 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