{"id":"cc4925e0-daf3-54d1-9eae-4f013c276552","stable_key":"aaa7baba-8689-56ab-ba1e-b71542bcb8e9:iodine-syn-acute-block-oxidation","predicate":"prevents","statement":"Methimazole suppressed the inhibitory effects of iodide on stimulated H2O2 generation in dog thyroid slices.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"ec07106b-b694-5cef-a187-b68a21ecbc6c","mechanism_event_label":"Preventing iodide oxidation blocked this feedback effect in thyroid tissue.","subject":{"id":"a3a516d1-65de-5638-8baa-bf4571450959","slug":"methimazole","display_name":"Methimazole","entity_type_key":"small_molecule"},"object":{"id":"11b58e47-fd03-5387-99f3-d2e29d47b3c3","slug":"iodide-induced-peroxide-inhibition","display_name":"Iodide induced peroxide inhibition","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"ec07106b-b694-5cef-a187-b68a21ecbc6c","stable_key":"aaa7baba-8689-56ab-ba1e-b71542bcb8e9:iodine-syn-acute-block-oxidation-event","event_type":"biochemical_relationship","label":"Preventing iodide oxidation blocked this feedback effect in thyroid tissue.","description":"Methimazole suppressed the inhibitory effects of iodide on stimulated H2O2 generation in dog thyroid slices.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"83b11ca6-52c7-5a1b-8c39-d9cdf89244e3","slug":"iodide","display_name":"Iodide ion","entity_type_key":"ion"},"role":"feedback_trigger","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"da9d64bc-69d4-5d97-90a4-8f0ed03e0ac8","slug":"hydrogen-peroxide","display_name":"Hydrogen peroxide","entity_type_key":"small_molecule"},"role":"measured_product","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"a3a516d1-65de-5638-8baa-bf4571450959","slug":"methimazole","display_name":"Methimazole","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"11b58e47-fd03-5387-99f3-d2e29d47b3c3","slug":"iodide-induced-peroxide-inhibition","display_name":"Iodide induced peroxide inhibition","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/iodine-synthesis-sources/2841932.json\", \"json_field\": \"abstractText\", \"text_sha256\": \"00e19301656f8e8fdb9a047cfa29daf1c66f9a73c84d6c450ff805a6d8b497eb\", \"text_characters\": 1113, \"note\": \"Exact publisher passage retained in the cited local source cache; locator and digest supplied here.\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Dog thyroid slices with iodide, stimulatory probes and methimazole","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Iodide exposure in dog thyroid slices stimulated by thyrotropin, carbamylcholine or intracellular-signaling probes; concentrations not given in abstract. Methimazole coexposure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Pharmacological probe supports oxidation dependence; the inhibitory iodinated species was not identified.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Iodine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"iodine","display_name":"Iodine","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Canis lupus familiaris","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Preventing iodide oxidation blocked this feedback effect in thyroid tissue.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[iodine-syn-block1988] Inhibition by iodide of iodide binding to proteins: the \"Wolff-Chaikoff\" effect is caused by inhibition of H2O2 generation. (1988). https://pubmed.ncbi.nlm.nih.gov/2841932/ DOI: 10.1016/0006-291x(88)90279-3","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Thyroid slices","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b9e69632-d609-5503-9457-24d6edbce52d","evidence_kind":"source_excerpt","locator":"Lines 814-826","start_line":814,"end_line":826,"excerpt":"### iodine-syn-acute-block-oxidation\nMethimazole suppressed the inhibitory effects of iodide on stimulated H2O2 generation in dog thyroid slices.\nCondition category: normal\nnutrient_topic: Iodine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Preventing iodide oxidation blocked this feedback effect in thyroid tissue.\norganism: Canis lupus familiaris\ntissue_or_cell_type: Thyroid slices\nexperimental_model: Dog thyroid slices with iodide, stimulatory probes and methimazole\nlimitations: Pharmacological probe supports oxidation dependence; the inhibitory iodinated species was not identified.\nexposure: Iodide exposure in dog thyroid slices stimulated by thyrotropin, carbamylcholine or intracellular-signaling probes; concentrations not given in abstract. Methimazole coexposure.\ncross_nutrient: false\nevidence_span: {\"source_cache\": \"artifacts/iodine-synthesis-sources/2841932.json\", \"json_field\": \"abstractText\", \"text_sha256\": \"00e19301656f8e8fdb9a047cfa29daf1c66f9a73c84d6c450ff805a6d8b497eb\", \"text_characters\": 1113, \"note\": \"Exact publisher passage retained in the cited local source cache; locator and digest supplied here.\"}\n[iodine-syn-block1988] Inhibition by iodide of iodide binding to proteins: the \"Wolff-Chaikoff\" effect is caused by inhibition of H2O2 generation. (1988). https://pubmed.ncbi.nlm.nih.gov/2841932/ DOI: 10.1016/0006-291x(88)90279-3","model_system":"Dog thyroid slices with iodide, stimulatory probes and methimazole","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [iodine-syn-block1988] Inhibition by iodide of iodide binding to proteins: the \"Wolff-Chaikoff\" effect is caused by inhibition of H2O2 generation. (1988). https://pubmed.ncbi.nlm.nih.gov/2841932/ DOI: 10.1016/0006-291x(88)90279-3","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"76da623d-a34a-5a5c-a942-d7571a85c486","stable_key":"import-aaa7baba-8689-56ab-ba1e-b71542bcb8e9","title":"Iodine: thyroid hormone production, deficiency, excess and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"a4885eb6f58bad4a4514c958b63834619e57b10aa7f694aa97d0f65c7d36548f","revision_id":"ea8001a1-b576-5eda-a193-883c7ad59b73","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}