{"id":"cc1cd550-6272-5301-8e5b-dff3375ebb3d","stable_key":"fd7915ff-856d-56c2-a118-4989693a7644:gingerols-6-neutrophil-pka","predicate":"increases","statement":"6-Gingerol enhanced measured PKA activity in the stimulated human-neutrophil experiments.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"9f7acc69-dda9-5f65-a957-3f0e65b3af57","mechanism_event_label":"6-Gingerol enhanced measured PKA activity in the stimulated human-neutrophil experiments.","subject":{"id":"837fdd65-c1eb-52e0-a724-42a1e9065c82","slug":"6-gingerol","display_name":"6-Gingerol","entity_type_key":"small_molecule"},"object":{"id":"88bc75dd-4f93-53d7-a023-8a92d821ce94","slug":"human-neutrophil-pka-activity","display_name":"Protein kinase A activity in human neutrophils","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"9f7acc69-dda9-5f65-a957-3f0e65b3af57","stable_key":"fd7915ff-856d-56c2-a118-4989693a7644:gingerols-6-neutrophil-pka-event","event_type":"observed_relationship","label":"6-Gingerol enhanced measured PKA activity in the stimulated human-neutrophil experiments.","description":"6-Gingerol enhanced measured PKA activity in the stimulated human-neutrophil experiments.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"837fdd65-c1eb-52e0-a724-42a1e9065c82","slug":"6-gingerol","display_name":"6-Gingerol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"88bc75dd-4f93-53d7-a023-8a92d821ce94","slug":"human-neutrophil-pka-activity","display_name":"Protein kinase A activity in human neutrophils","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"6cd7f461-9b79-5f75-877f-99910bb84f6c","slug":"pka","display_name":"Protein kinase A","entity_type_key":"protein_family"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"9cc3323e-3a7f-5be5-91ae-78dab284fb81","slug":"cyclic-amp","display_name":"Cyclic adenosine monophosphate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"6-Gingerol 10 micromolar with forskolin or cAMP stimulation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Kinase assay incubation 90 min; cellular pretreatment interval not resolved here","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary open full text, results, figure legends and methods, plus PubMed metadata.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation, not universally established human effects","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Neutrophils isolated from healthy human donors","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Activity response, not demonstrated direct binding of gingerol to PKA.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Gingerols collection; each molecular form and experimental preparation remains explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"gingerols","display_name":"Gingerols","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Neutrophils isolated from healthy human donors","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"6-Gingerol enhanced measured PKA activity in the stimulated human-neutrophil experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Antineutrophil properties of natural gingerols in models of lupus. 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