{"id":"cbe70590-4232-51f8-ab34-bd2c3131896f","stable_key":"708c252b-0aef-5cec-95bf-99d19578214c:apt2-s122a-stat3-palmit","predicate":"has_contextual_experimental_result","statement":"APT2-S122A expression did not reproduce the wild-type reduction in STAT3 palmitoylation.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"7c4b1b7f-39fb-5039-84d7-9d22443de249","mechanism_event_label":"apt2-s122a-stat3-palmit","subject":{"id":"9af4676b-84d9-5bfd-a15a-8b647f27353f","slug":"human-apt2-s122a-expression-hek293t","display_name":"Human APT2 S122A expression in HEK293T","entity_type_key":"protein_state"},"object":{"id":"2bb7e799-f77a-5a72-b8d6-3ec458cc11aa","slug":"human-stat3-palmitoylation","display_name":"STAT3 palmitoylation in human cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"7c4b1b7f-39fb-5039-84d7-9d22443de249","stable_key":"708c252b-0aef-5cec-95bf-99d19578214c:apt2-s122a-stat3-palmit","event_type":"experimental_result","label":"apt2-s122a-stat3-palmit","description":"APT2-S122A expression did not reproduce the wild-type reduction in STAT3 palmitoylation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9af4676b-84d9-5bfd-a15a-8b647f27353f","slug":"human-apt2-s122a-expression-hek293t","display_name":"Human APT2 S122A expression in HEK293T","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2bb7e799-f77a-5a72-b8d6-3ec458cc11aa","slug":"human-stat3-palmitoylation","display_name":"STAT3 palmitoylation in human cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"f2ffef5b-5457-50a8-b787-b8f24430f610","slug":"lypla2","display_name":"LYPLA2","entity_type_key":"protein"},"role":"expressed protein","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"dc129d3d-aaa0-5511-9a60-3e3880b0eb0f","slug":"stat3","display_name":"STAT3","entity_type_key":"protein"},"role":"measured protein","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Human HEK293T with expressed APT2 and Flag-STAT3","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Expression constructs; 50 micromolar Alk14 labeling for 5 hours; expression duration unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"C2S disrupts the APT2 palmitoylation site; S122A alters its catalytic site. They are not interchangeable controls. Null results do not establish equivalence. GPX4 was not an endpoint.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Human","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_locator","value_text":"Figure 2d; Extended Data 7b-c.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"https://doi.org/10.1038/s41586-020-2799-2","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f94f1bb4-4dfb-575e-b954-20fa897ee563","evidence_kind":"source_excerpt","locator":"Lines 60-66","start_line":60,"end_line":66,"excerpt":"APT2-S122A expression did not reproduce the wild-type reduction in STAT3 palmitoylation.\nprimary_references: https://doi.org/10.1038/s41586-020-2799-2\nprimary_locator: Figure 2d; Extended Data 7b-c.\norganism: Human\nexperimental_model: Human HEK293T with expressed APT2 and Flag-STAT3\nexposure: Expression constructs; 50 micromolar Alk14 labeling for 5 hours; expression duration unresolved.\nlimitations: C2S disrupts the APT2 palmitoylation site; S122A alters its catalytic site. They are not interchangeable controls. Null results do not establish equivalence. GPX4 was not an endpoint.","model_system":"Human HEK293T with expressed APT2 and Flag-STAT3","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Curator paraphrase of the specified primary result; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"01fc674f-07c6-58fd-b1e7-877478a4472e","stable_key":"import-708c252b-0aef-5cec-95bf-99d19578214c","title":"APT2-STAT3 cycling and a competing sulforaphane target route","document_type":"imported_text","citation_label":"Selected primary observations: 10.1038/s41586-020-2799-2 and 10.1038/s41388-020-1335-z.","file_path":"","sha256":"b1b245468dac7acc94a2fef690d994124b2bc4fc5f92135173cf5636af391395","revision_id":"d84b8419-69dd-50d9-b587-5627ed414591","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}