{"id":"cbdb5312-883a-5bd4-8c05-84ffe27925de","stable_key":"8182578e-8d21-598e-a3cb-e62d480d2251:myricetin-mast-tryptase","predicate":"reduces","statement":"Myricetin inhibited tryptase release from anti-IgE-activated human mast cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"ffc96850-ed50-5077-bfe4-2bb6c18969fe","mechanism_event_label":"The allergic response involves multiple independently recorded steps.","subject":{"id":"fafe05e7-4874-56e6-a42a-2ebf1ab2dd57","slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"},"object":{"id":"f8fc8504-a5f9-5abd-adce-b2fd865377a1","slug":"human-mast-cell-tryptase-pool","display_name":"Human mast-cell tryptase, isoform unresolved","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"ffc96850-ed50-5077-bfe4-2bb6c18969fe","stable_key":"8182578e-8d21-598e-a3cb-e62d480d2251:myricetin-mast-tryptase-event","event_type":"observed_relationship","label":"The allergic response involves multiple independently recorded steps.","description":"Myricetin inhibited tryptase release from anti-IgE-activated human mast cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"fafe05e7-4874-56e6-a42a-2ebf1ab2dd57","slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f8fc8504-a5f9-5abd-adce-b2fd865377a1","slug":"human-mast-cell-tryptase-pool","display_name":"Human mast-cell tryptase, isoform unresolved","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human cord-blood-derived mast cells; 15-minute pretreatment, 0.01–100 micromolar tested.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No oral allergy trial; reduced phosphorylation is not direct kinase inhibition.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Myricetin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The allergic response involves multiple independently recorded steps.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Flavonols inhibit proinflammatory mediator release, intracellular calcium ion levels and protein kinase C theta phosphorylation in human mast cells. · 2005 · https://pubmed.ncbi.nlm.nih.gov/15912140/ · DOI 10.1038/sj.bjp.0706246","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"3edb4986-cc72-54f2-9a62-2f496a8a6cab","evidence_kind":"source_excerpt","locator":"Lines 428-434","start_line":428,"end_line":434,"excerpt":"## myricetin-mast-tryptase\nThe allergic response involves multiple independently recorded steps.\nMyricetin inhibited tryptase release from anti-IgE-activated human mast cells.\nModel: Human cord-blood-derived mast cells; 15-minute pretreatment, 0.01–100 micromolar tested.\nLimitations: No oral allergy trial; reduced phosphorylation is not direct kinase inhibition.\nEvidence access: Primary abstract\nFlavonols inhibit proinflammatory mediator release, intracellular calcium ion levels and protein kinase C theta phosphorylation in human mast cells. · 2005 · https://pubmed.ncbi.nlm.nih.gov/15912140/ · DOI 10.1038/sj.bjp.0706246","model_system":"Human cord-blood-derived mast cells; 15-minute pretreatment, 0.01–100 micromolar tested.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"58045cd9-3b27-5469-bb4b-e1c6a9bdca34","stable_key":"import-8182578e-8d21-598e-a3cb-e62d480d2251","title":"Myricetin: metabolism, immune signaling, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"0abf512275cf3aae9e4ec8544f1cde97e0155d0cfb5be12a193cdd5a898386a7","revision_id":"422e342f-a55c-5e73-ba98-43df560f49b9","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}