{"id":"cba9503e-c78b-5a16-a7dd-7181f0dcf198","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-d-inflammasome","predicate":"increases_tested","statement":"Cucurbitacin D enhanced LPS-associated IL-1-beta production and caspase-1-dependent inflammasome activation; the study reported NLRP3–ASC interaction and an ERK-dependent transcription component.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"39f99760-bc18-56a4-9de5-5b998413618d","mechanism_event_label":"This member increased an inflammatory response under these conditions.","subject":{"id":"13edf322-5d31-5a4e-802f-c7528fc2ef48","slug":"cucurbitacin-d","display_name":"Cucurbitacin D","entity_type_key":"small_molecule"},"object":{"id":"3c150e11-fc7c-5f44-ac0b-89bc24215556","slug":"cud-mixed-myeloid-inflammasome","display_name":"Cucurbitacin D inflammatory response in human/mouse myeloid study panel","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"39f99760-bc18-56a4-9de5-5b998413618d","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-d-inflammasome-event","event_type":"observed_relationship","label":"This member increased an inflammatory response under these conditions.","description":"Cucurbitacin D enhanced LPS-associated IL-1-beta production and caspase-1-dependent inflammasome activation; the study reported NLRP3–ASC interaction and an ERK-dependent transcription component.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"13edf322-5d31-5a4e-802f-c7528fc2ef48","slug":"cucurbitacin-d","display_name":"Cucurbitacin D","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3c150e11-fc7c-5f44-ac0b-89bc24215556","slug":"cud-mixed-myeloid-inflammasome","display_name":"Cucurbitacin D inflammatory response in human/mouse myeloid study panel","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Panel included human THP-1 and murine RAW264, peritoneal and bone-marrow-derived macrophages; individual assay species unresolved in accessed abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Mixed-model process is retained explicitly; no individual molecular result is silently assigned to human cells. Different member/stimulus from CuB A549 suppression.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Cucurbitacins collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"cucurbitacin","display_name":"Cucurbitacins","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"This member increased an inflammatory response under these conditions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Cucurbitacin D is a new inflammasome activator in macrophages. · 2013 · https://pubmed.ncbi.nlm.nih.gov/24140411/ · DOI 10.1016/j.intimp.2013.10.003","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6683ccba-768b-52c8-a619-75b843dc2ead","evidence_kind":"source_excerpt","locator":"Lines 340-346","start_line":340,"end_line":346,"excerpt":"## cucurbitacin-d-inflammasome\nThis member increased an inflammatory response under these conditions.\nCucurbitacin D enhanced LPS-associated IL-1-beta production and caspase-1-dependent inflammasome activation; the study reported NLRP3–ASC interaction and an ERK-dependent transcription component.\nModel: Panel included human THP-1 and murine RAW264, peritoneal and bone-marrow-derived macrophages; individual assay species unresolved in accessed abstract.\nLimitations: Mixed-model process is retained explicitly; no individual molecular result is silently assigned to human cells. Different member/stimulus from CuB A549 suppression.\nEvidence access: Primary abstract\nCucurbitacin D is a new inflammasome activator in macrophages. · 2013 · https://pubmed.ncbi.nlm.nih.gov/24140411/ · DOI 10.1016/j.intimp.2013.10.003","model_system":"Panel included human THP-1 and murine RAW264, peritoneal and bone-marrow-derived macrophages; individual assay species unresolved in accessed abstract.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"77324310-00ce-5d28-9fc7-595fddae86b9","stable_key":"import-2921131d-0ba0-51d3-8f59-8c4be3b9ac08","title":"Cucurbitacins: thiol chemistry, cytoskeleton, metabolic dependencies and signaling (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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