{"id":"cb9cbaf5-22a5-5f70-9e55-abcd25f3a52b","stable_key":"33bef151-bdc7-5892-aff6-0345ab149739:ace-inhibition-does-not-require-the-intact-enzyme","predicate":"is_not_required_for_the_inhibition_of","statement":"Catalytically active intact nattokinase is not required for the ACE-inhibitory activity of the material, because heat inactivation raises rather than removes it.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"2e76ca8c-a49f-52e6-b80b-e26e8578b6b3","mechanism_event_label":"Catalytically active intact nattokinase is not required for the ACE-inhibitory activity of the material, because heat inactivation raises rather than removes it.","subject":{"id":"6b03a125-209a-5060-8b35-ec9e0e54033c","slug":"nattokinase","display_name":"Nattokinase / subtilisin NAT","entity_type_key":"peptide"},"object":{"id":"3d4b5a5c-1666-5b4a-b4e7-60bc6de0e626","slug":"ace","display_name":"Human angiotensin-converting enzyme / ACE","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"2e76ca8c-a49f-52e6-b80b-e26e8578b6b3","stable_key":"33bef151-bdc7-5892-aff6-0345ab149739:ace-inhibition-does-not-require-the-intact-enzyme-event","event_type":"biochemical_relationship","label":"Catalytically active intact nattokinase is not required for the ACE-inhibitory activity of the material, because heat inactivation raises rather than removes it.","description":"ACE inhibition assay","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6b03a125-209a-5060-8b35-ec9e0e54033c","slug":"nattokinase","display_name":"Nattokinase / subtilisin NAT","entity_type_key":"peptide"},"role":"acting component","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3d4b5a5c-1666-5b4a-b4e7-60bc6de0e626","slug":"ace","display_name":"Human angiotensin-converting enzyme / ACE","entity_type_key":"protein"},"role":"component the finding is about","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"duration","value_text":"Not stated here","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified subtilisin NAT","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Active compared with heat-inactivated subtilisin NAT","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This is a statement about one activity only. Every direct-proteolysis mechanism in this chapter does require the intact active enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Purified subtilisin NAT","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Catalytically active intact nattokinase is not required for the ACE-inhibitory activity of the material, because heat inactivation raises rather than removes it.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Inhibition of angiotensin I converting enzyme by subtilisin NAT (nattokinase) in natto, a Japanese traditional fermented food. 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Every reference in that document was resolved against live PubMed with its abstract read and its DOI cross-checked on 2026-09-23, and the EFSA novel-food opinion was retrieved and read in full. That check corrected two PMIDs that pointed at unrelated papers, two DOIs, and two papers recorded as carrying no erratum that do carry one; it also reversed three findings the supplied document had stated backwards. Two papers carry a published correction, recorded as such and not as a retraction. Three sources are not indexed in PubMed and are cited by what they have. Laboratory lineages are recorded, so the four papers from one group, the three from another and the two readings of a single applicant dossier cannot be counted as separate lines of support. Study-specific doses, units, populations and limitations retained; activity units are never converted between systems. Not publisher full text.","file_path":"","sha256":"34fc46e0e849d06bfa13cb099a79b50679dd6fd0de8a5a0575326afdde3259c1","revision_id":"efd063e6-e982-5f6e-a4b8-6f2ffe4185ce","review_status":"unverified_draft","notes":""}}],"relations":[{"id":"6e9703ea-c101-5eab-8531-8f9e3fb7d44d","relation_type":"limits_scope_of","notes":"The human blood-pressure signal cannot be assigned to the enzyme while a peptide route exists that does not need it.","source_claim_id":"cb9cbaf5-22a5-5f70-9e55-abcd25f3a52b","source_claim_key":"33bef151-bdc7-5892-aff6-0345ab149739:ace-inhibition-does-not-require-the-intact-enzyme","source_statement":"Catalytically active intact nattokinase is not required for the ACE-inhibitory activity of the material, because heat inactivation raises rather than removes it.","target_claim_id":"b540bd62-dbd2-557b-a0b4-fb1ab06f535e","target_claim_key":"33bef151-bdc7-5892-aff6-0345ab149739:eight-weeks-of-nsk-sd-lowers-blood-pressure","target_statement":"Net changes of -5.55 mmHg systolic and -2.84 mmHg diastolic against placebo were reported after eight weeks at 2,000 FU per day."}],"conflicts":[],"corrections":[],"research":null}