{"id":"cb9bc20a-53c7-545c-b2d3-be3003bd7a47","stable_key":"27e0c1cf-7726-5164-8b15-27a631584cd0:choline-cht1-recycling","predicate":"reimports","statement":"CHT1 supports reutilization of choline liberated by synaptic acetylcholine hydrolysis, sustaining presynaptic acetylcholine synthesis.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"97b9202f-064d-523c-a4e1-7120bd0d20ff","mechanism_event_label":"After acetylcholine is broken down, choline can be taken back up and reused.","subject":{"id":"04646285-c618-515d-8baa-57f7805cf6c0","slug":"slc5a7","display_name":"Human high-affinity choline transporter CHT1 / SLC5A7","entity_type_key":"protein"},"object":{"id":"8e1d19fd-ad66-575d-81c4-9122f78915e5","slug":"choline","display_name":"Choline","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"97b9202f-064d-523c-a4e1-7120bd0d20ff","stable_key":"27e0c1cf-7726-5164-8b15-27a631584cd0:choline-cht1-recycling-event","event_type":"biochemical_relationship","label":"After acetylcholine is broken down, choline can be taken back up and reused.","description":"CHT1 supports reutilization of choline liberated by synaptic acetylcholine hydrolysis, sustaining presynaptic acetylcholine synthesis.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"564da968-7b87-55cf-b324-222979314e45","slug":"acetylcholine","display_name":"Acetylcholine","entity_type_key":"small_molecule"},"role":"upstream_source","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"04646285-c618-515d-8baa-57f7805cf6c0","slug":"slc5a7","display_name":"Human high-affinity choline transporter CHT1 / SLC5A7","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8e1d19fd-ad66-575d-81c4-9122f78915e5","slug":"choline","display_name":"Choline","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/choline-research/38589607.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"3cf951acec5056180aacccf73a96951c54fa40be5446cd778bd90eb23b43fd3a\", \"start_char\": 0, \"end_char\": 1090, \"text_sha256\": \"3cf951acec5056180aacccf73a96951c54fa40be5446cd778bd90eb23b43fd3a\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human CHT1 cryo-EM and functional characterization","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Choline-bound, inhibitor-bound and apo conformations","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Structural transport cycle; no claim of cognitive benefit from increasing intake.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Choline research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"choline","display_name":"Choline","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human CHT1 protein","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"After acetylcholine is broken down, choline can be taken back up and reused.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[choline-p38589607] Transport mechanism of presynaptic high-affinity choline uptake by CHT1. (2024). https://pubmed.ncbi.nlm.nih.gov/38589607/ DOI: 10.1038/s41594-024-01259-w","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Presynaptic uptake mechanism and experimental structures","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"84c1c669-c4d2-5f8e-bfd8-d37b6d4aadbb","evidence_kind":"source_excerpt","locator":"Lines 334-345","start_line":334,"end_line":345,"excerpt":"### choline-cht1-recycling\nCHT1 supports reutilization of choline liberated by synaptic acetylcholine hydrolysis, sustaining presynaptic acetylcholine synthesis.\nCondition category: normal\nnutrient_topic: Choline research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: After acetylcholine is broken down, choline can be taken back up and reused.\norganism: Human CHT1 protein\ntissue_or_cell_type: Presynaptic uptake mechanism and experimental structures\nexperimental_model: Human CHT1 cryo-EM and functional characterization\nlimitations: Structural transport cycle; no claim of cognitive benefit from increasing intake.\nexposure: Choline-bound, inhibitor-bound and apo conformations\nevidence_span: {\"source_cache\": \"artifacts/choline-research/38589607.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"3cf951acec5056180aacccf73a96951c54fa40be5446cd778bd90eb23b43fd3a\", \"start_char\": 0, \"end_char\": 1090, \"text_sha256\": \"3cf951acec5056180aacccf73a96951c54fa40be5446cd778bd90eb23b43fd3a\"}\n[choline-p38589607] Transport mechanism of presynaptic high-affinity choline uptake by CHT1. 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