{"id":"cb7cef1a-faef-576d-a11d-37b6f615868c","stable_key":"434e1e27-eb90-583a-956b-62472726ffba:ca-orai1-calcium-permeation","predicate":"transports","statement":"ORAI1 supplies the calcium-selective CRAC pore; transmembrane substitutions alter calcium and monovalent-ion permeability.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"7c5e001d-e9a5-512b-9f92-704bbe0a6257","mechanism_event_label":"ORAI1 forms the pore through which store-operated calcium enters.","subject":{"id":"c214f817-afc5-567e-ae17-ab5558e88394","slug":"orai1","display_name":"ORAI1","entity_type_key":"protein"},"object":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"evidence_count":1,"mechanism_event":{"id":"7c5e001d-e9a5-512b-9f92-704bbe0a6257","stable_key":"434e1e27-eb90-583a-956b-62472726ffba:ca-orai1-calcium-permeation-event","event_type":"transport","label":"ORAI1 forms the pore through which store-operated calcium enters.","description":"ORAI1 supplies the calcium-selective CRAC pore; transmembrane substitutions alter calcium and monovalent-ion permeability.","status":"provisional","compartment":{"slug":"plasma-membrane","display_name":"Plasma membrane"},"participants":[{"entity":{"id":"c214f817-afc5-567e-ae17-ab5558e88394","slug":"orai1","display_name":"ORAI1","entity_type_key":"protein"},"role":"channel","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"transported_substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"f0b6bcb6-5d41-5d9d-af9f-6af1a94d5c5a","slug":"plasma-membrane","display_name":"Plasma membrane","entity_type_key":"organelle"},"role":"experimental_location","stoichiometry":null,"state_label":"","sequence_order":2,"notes":"Study location; presence here is not a separate transport or causal claim."}]},"contexts":[{"dimension":"compartment_description","value_text":"Plasma membrane","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human ORAI1 mutagenesis and membrane-current/selectivity measurements","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Pore evidence does not specify channel stoichiometry or all tissue contributions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Calcium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"calcium","display_name":"Calcium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"ORAI1 forms the pore through which store-operated calcium enters.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ca-prakriya2006] Orai1 is an essential pore subunit of the CRAC channel (2006). https://www.nature.com/articles/nature05122 DOI: 10.1038/nature05122","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"research_relationship_category","value_text":"transport","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Recombinant channel assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_effect","value_text":"raises","comparator":null,"unit":null,"notes":"The CRAC pore carries calcium from extracellular fluid to cytosol.","entity":null},{"dimension":"transport_or_reaction_direction","value_text":"Extracellular fluid to cytosol","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_pool","value_text":"cytosolic calcium","comparator":null,"unit":null,"notes":"The CRAC pore carries calcium from extracellular fluid to cytosol.","entity":null}],"evidence":[{"id":"76505471-bef5-589f-a97b-ca63becd15d2","evidence_kind":"source_excerpt","locator":"Lines 544-556","start_line":544,"end_line":556,"excerpt":"### ca-orai1-calcium-permeation\nORAI1 supplies the calcium-selective CRAC pore; transmembrane substitutions alter calcium and monovalent-ion permeability.\nCondition category: normal\nnutrient_topic: Calcium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: ORAI1 forms the pore through which store-operated calcium enters.\norganism: Homo sapiens\ntissue_or_cell_type: Recombinant channel assays\nexperimental_model: Human ORAI1 mutagenesis and membrane-current/selectivity measurements\nlimitations: Pore evidence does not specify channel stoichiometry or all tissue contributions.\nresearch_relationship_category: transport\ntransport_or_reaction_direction: Extracellular fluid to cytosol\ncompartment_description: Plasma membrane\n[ca-prakriya2006] Orai1 is an essential pore subunit of the CRAC channel (2006). https://www.nature.com/articles/nature05122 DOI: 10.1038/nature05122","model_system":"Human ORAI1 mutagenesis and membrane-current/selectivity measurements","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [ca-prakriya2006] Orai1 is an essential pore subunit of the CRAC channel (2006). https://www.nature.com/articles/nature05122 DOI: 10.1038/nature05122","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3c083fa0-59c6-50e9-af42-678d0e3c1006","stable_key":"import-434e1e27-eb90-583a-956b-62472726ffba","title":"Calcium: mechanism-first literature curation (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"965c934d6b147f2b62f8d45ce6d7a87681a600bfe5c46a585b4704889afd3fc1","revision_id":"543ec48b-4f70-5197-9d76-f5a3c443664c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}