{"id":"cb540a46-e7b4-5690-a012-56a56f9678ae","stable_key":"6d38d43e-01e4-5641-93be-65654271e242:zn-sig-luminal-copper-zinc","predicate":"high_luminal_exposure_reduced","statement":"High luminal copper increased mucosal zinc accumulation while reducing zinc transfer to portal effluent at the highest luminal zinc concentration.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"341590c2-d754-5268-8913-f8408d438f59","mechanism_event_label":"Copper altered zinc movement through, as well as into, intestinal cells.","subject":{"id":"9f0afdde-1ec1-5c8a-bb5e-f3b2b75f67f6","slug":"copper","display_name":"Copper","entity_type_key":"nutrient_element"},"object":{"id":"957321e4-a5db-5a5d-851b-72069bab3efd","slug":"intestinal-zinc-absorption","display_name":"Intestinal zinc absorption","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"341590c2-d754-5268-8913-f8408d438f59","stable_key":"6d38d43e-01e4-5641-93be-65654271e242:zn-sig-luminal-copper-zinc-event","event_type":"biochemical_relationship","label":"Copper altered zinc movement through, as well as into, intestinal cells.","description":"High luminal copper increased mucosal zinc accumulation while reducing zinc transfer to portal effluent at the highest luminal zinc concentration.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"0429f0ff-c033-514b-9a4f-6f0f83268095","slug":"zinc","display_name":"Zinc","entity_type_key":"nutrient_element"},"role":"affected_nutrient","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9f0afdde-1ec1-5c8a-bb5e-f3b2b75f67f6","slug":"copper","display_name":"Copper","entity_type_key":"nutrient_element"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"957321e4-a5db-5a5d-851b-72069bab3efd","slug":"intestinal-zinc-absorption","display_name":"Intestinal zinc absorption","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"Zinc (affected_nutrient)","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/zinc-signaling-sources/3968585-abstract.txt\", \"locator\": \"Primary indexed abstract\", \"file_sha256\": \"ca588cec5326d23dd5f8c9b409ea66fc415043b95af456399e495b8131c8f6ed\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Isolated vascularly perfused intestine from diet-conditioned rats","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Dietary copper/zinc variation for one week versus direct luminal-metal variation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Ex-vivo perfusion and extreme luminal exposure differ from whole-animal feeding; increased metallothionein-bound copper alone did not prove reduced net absorption.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Zinc research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"zinc","display_name":"Zinc","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Rattus norvegicus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Copper altered zinc movement through, as well as into, intestinal cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[zn-sig-3968585] Copper and zinc absorption in the rat: mechanism of mutual antagonism. (1985). https://pubmed.ncbi.nlm.nih.gov/3968585/ DOI: 10.1093/jn/115.2.159","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Small-intestinal mucosa and portal effluent","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"d9f5bc40-e559-5f14-b57d-d092f817558e","evidence_kind":"source_excerpt","locator":"Lines 971-983","start_line":971,"end_line":983,"excerpt":"### zn-sig-luminal-copper-zinc\nHigh luminal copper increased mucosal zinc accumulation while reducing zinc transfer to portal effluent at the highest luminal zinc concentration.\nCondition category: normal\nnutrient_topic: Zinc research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Copper altered zinc movement through, as well as into, intestinal cells.\norganism: Rattus norvegicus\ntissue_or_cell_type: Small-intestinal mucosa and portal effluent\nexperimental_model: Isolated vascularly perfused intestine from diet-conditioned rats\nlimitations: Ex-vivo perfusion and extreme luminal exposure differ from whole-animal feeding; increased metallothionein-bound copper alone did not prove reduced net absorption.\nexposure: Dietary copper/zinc variation for one week versus direct luminal-metal variation.\ncross_nutrient: Zinc (affected_nutrient)\nevidence_span: {\"source_cache\": \"artifacts/zinc-signaling-sources/3968585-abstract.txt\", \"locator\": \"Primary indexed abstract\", \"file_sha256\": \"ca588cec5326d23dd5f8c9b409ea66fc415043b95af456399e495b8131c8f6ed\"}\n[zn-sig-3968585] Copper and zinc absorption in the rat: mechanism of mutual antagonism. 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