{"id":"cb44fb80-be88-59b2-b343-41270de4a865","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-b-tlr4-engagement","predicate":"has_supported_cellular_engagement_with","statement":"Docking and CETSA supported an interaction between cucurbitacin B and TLR4 in the NSCLC study.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"32188dcd-53fe-51d9-8eb1-2f72c6d7b421","mechanism_event_label":"Another proposed target was tested in cells.","subject":{"id":"a544a561-7647-55f6-8ba1-c7ab0133f968","slug":"cucurbitacin-b","display_name":"Cucurbitacin B","entity_type_key":"small_molecule"},"object":{"id":"3f792f3c-3129-5995-8341-2295c5ea5b27","slug":"tlr4","display_name":"Human toll-like receptor 4 / TLR4","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"32188dcd-53fe-51d9-8eb1-2f72c6d7b421","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-b-tlr4-engagement-event","event_type":"observed_relationship","label":"Another proposed target was tested in cells.","description":"Docking and CETSA supported an interaction between cucurbitacin B and TLR4 in the NSCLC study.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a544a561-7647-55f6-8ba1-c7ab0133f968","slug":"cucurbitacin-b","display_name":"Cucurbitacin B","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3f792f3c-3129-5995-8341-2295c5ea5b27","slug":"tlr4","display_name":"Human toll-like receptor 4 / TLR4","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human NSCLC cell experiments with animal tumor models in the same paper.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No purified affinity or exclusive target assignment follows from docking plus CETSA.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Cucurbitacins collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"cucurbitacin","display_name":"Cucurbitacins","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"Another proposed target was tested in cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Cucurbitacin B inhibits non-small cell lung cancer in vivo and in vitro by triggering TLR4/NLRP3/GSDMD-dependent pyroptosis. · 2021 · https://pubmed.ncbi.nlm.nih.gov/34217831/ · DOI 10.1016/j.phrs.2021.105748","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"68d85baa-752e-57a8-9bcf-919f00544b7d","evidence_kind":"source_excerpt","locator":"Lines 284-290","start_line":284,"end_line":290,"excerpt":"## cucurbitacin-b-tlr4-engagement\nAnother proposed target was tested in cells.\nDocking and CETSA supported an interaction between cucurbitacin B and TLR4 in the NSCLC study.\nModel: Human NSCLC cell experiments with animal tumor models in the same paper.\nLimitations: No purified affinity or exclusive target assignment follows from docking plus CETSA.\nEvidence access: Primary abstract\nCucurbitacin B inhibits non-small cell lung cancer in vivo and in vitro by triggering TLR4/NLRP3/GSDMD-dependent pyroptosis. · 2021 · https://pubmed.ncbi.nlm.nih.gov/34217831/ · DOI 10.1016/j.phrs.2021.105748","model_system":"Human NSCLC cell experiments with animal tumor models in the same paper.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"77324310-00ce-5d28-9fc7-595fddae86b9","stable_key":"import-2921131d-0ba0-51d3-8f59-8c4be3b9ac08","title":"Cucurbitacins: thiol chemistry, cytoskeleton, metabolic dependencies and signaling (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"5c23ec61b9cf3d2966fb081d8617e1e1f7c522e86419b8ba2dcae09590121123","revision_id":"74251a06-3ba5-506c-b428-700eca0fb432","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}