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(2015). https://pubmed.ncbi.nlm.nih.gov/25843658/ DOI: 10.1016/j.freeradbiomed.2015.03.016","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Embryonic fibroblasts","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"9cf75b3a-2c8f-5cf2-a730-56f9449b6265","evidence_kind":"source_excerpt","locator":"Lines 718-730","start_line":718,"end_line":730,"excerpt":"### ver-gpx4-fibroblast-repair-rescue\nIn mouse embryonic fibroblasts subjected to 48-hour inducible Gpx4 deletion, 200 µM alpha-tocopherol supplementation for the preceding 24 hours prevented the pronounced laser-wound repair defect.\nCondition category: machinery_impairment\nnutrient_topic: Vitamin E research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Vitamin E loading rescued membrane resealing after GPX4 deletion in these fibroblasts.\norganism: Mus musculus\ntissue_or_cell_type: Embryonic fibroblasts\nexperimental_model: Tamoxifen-inducible Gpx4 deletion with re-expression controls\nlimitations: Genetic GPX4 loss, not nutritional selenium deficiency; repair mechanism downstream of oxidation was not resolved.\nexposure: 1 µM tamoxifen for 48 h; 200 µM alpha-tocopherol for 24 h before repair assay.\ncross_nutrient: true\nevidence_location: Figure 4d; Methods\n[ver-labazi2015] The antioxidant requirement for plasma membrane repair in skeletal muscle. 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